State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China.
Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei, China.
J Sci Food Agric. 2020 Jan 15;100(1):161-167. doi: 10.1002/jsfa.10009. Epub 2019 Oct 8.
Rapid and accurate diagnosis of nitrogen (N) status in field crops is of great significance for site-specific N fertilizer management. This study aimed to evaluate the potential of hyperspectral imaging coupled with chemometrics for the qualitative and quantitative diagnosis of N status in tea plants under field conditions.
Hyperspectral data from mature leaves of tea plants with different N application rates were preprocessed by standard normal variate (SNV). Partial least squares discriminative analysis (PLS-DA) and least squares-support vector machines (LS-SVM) were used for the classification of different N status. Furthermore, partial least squares regression (PLSR) was used for the prediction of N content. The results showed that the LS-SVM model yielded better performance with correct classification rates of 82% and 92% in prediction sets for the diagnosis of different N application rates and N status, respectively. The PLSR model for leaf N content (LNC) showed excellent performance, with correlation coefficients of 0.924, root mean square error of 0.209, and residual predictive deviation of 2.686 in the prediction set. In addition, the important wavebands of the PLSR model were interpreted based on regression coefficients.
Overall, our results suggest that the hyperspectral imaging technique can be an effective and accurate tool for qualitative and quantitative diagnosis of N status in tea plants. © 2019 Society of Chemical Industry.
快速准确地诊断田间作物的氮(N)状况对于实施基于地点的氮肥管理具有重要意义。本研究旨在评估高光谱成像技术与化学计量学相结合在现场条件下定性和定量诊断茶树 N 状况的潜力。
对不同施氮量的茶树成熟叶片的高光谱数据进行标准正态变量(SNV)预处理。采用偏最小二乘判别分析(PLS-DA)和最小二乘支持向量机(LS-SVM)对不同 N 状况进行分类。此外,还采用偏最小二乘回归(PLSR)对 N 含量进行预测。结果表明,LS-SVM 模型的性能更好,在预测集对不同施氮量和 N 状况的分类中,正确分类率分别为 82%和 92%。叶片 N 含量(LNC)的 PLSR 模型表现出优异的性能,在预测集中相关系数为 0.924,均方根误差为 0.209,剩余预测偏差为 2.686。此外,根据回归系数对 PLSR 模型的重要波段进行了解释。
总体而言,我们的研究结果表明,高光谱成像技术可以成为一种有效且准确的工具,用于定性和定量诊断茶树的 N 状况。 © 2019 英国化学学会。