Departament de Genètica i Microbiologia. Facultat de Biociències. Universitat Autònoma de Barcelona. Bellaterra, Cerdanyola del Vallès, 08193 Barcelona, Spain.
Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193 Barcelona, Spain.
Chemosphere. 2020 Jan;238:124663. doi: 10.1016/j.chemosphere.2019.124663. Epub 2019 Aug 24.
Ochrobactrum anthropi DE2010 is a microorganism isolated from Ebro Delta microbial mats and able to resist high doses of chromium(III) due to its capacity to tolerate, absorb and accumulate this metal. The effect of this pollutant on O. anthropi DE2010 has been studied assessing changes in viability and biomass, sorption yields and removal efficiencies. Furthermore, and for the first time, its capacity for immobilizing Cr(III) from culture media was tested by a combination of High Angle Annular Dark Field (HAADF) Scanning Transmission Electron Microscopy (STEM) imaging coupled to Energy Dispersive X-ray spectroscopy (EDX). The results showed that O. anthropi DE2010 was grown optimally at 0-2 mM Cr(III). On the other hand, from 2 to 10 mM Cr(III) microbial plate counts, growth rates, cell viability, and biomass decreased while extracellular polymeric substances (EPS) production increases. Furthermore, this bacterium had a great ability to remove Cr(III) at 10 mM (q = 950.00 mg g) immobilizing it mostly in bright polyphosphate inclusions and secondarily on the cellular surface at the EPS level. Based on these results, O. anthropi DE2010 could be considered as a potential agent for bioremediation in Cr(III) contaminated environments.
耐铬(III)欧文氏菌 DE2010 是从埃布罗三角洲微生物垫中分离出来的一种微生物，由于其耐受、吸收和积累这种金属的能力，能够抵抗高剂量的铬(III)。研究了这种污染物对 O. anthropi DE2010 的影响，评估了其活力和生物量、吸附产率和去除效率的变化。此外，首次通过高角度环形暗场 (HAADF) 扫描透射电子显微镜 (STEM) 成像与能量色散 X 射线光谱 (EDX) 相结合，测试了其从培养基中固定 Cr(III)的能力。结果表明，O. anthropi DE2010 在 0-2 mM Cr(III)下生长最佳。另一方面，从 2 到 10 mM Cr(III)时，微生物平板计数、生长速率、细胞活力和生物量下降，而细胞外多聚物 (EPS) 产量增加。此外，该细菌在 10 mM 时具有很强的去除 Cr(III)的能力(q = 950.00 mg g)，主要将其固定在亮的多磷酸盐包含物中，其次在 EPS 水平上固定在细胞表面。基于这些结果，O. anthropi DE2010 可以被认为是 Cr(III)污染环境生物修复的潜在剂。
