State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences, Fuzhou 350002, China.
J Environ Sci (China). 2009;21(12):1673-8. doi: 10.1016/s1001-0742(08)62472-9.
A Gram-negative, chromium(VI) tolerant and reductive strain CTS-325, isolated from a Chinese chromate plant, was identified as Ochrobactrum anthropi based on its biochemical properties and 16S rDNA sequence analysis. It was able to tolerate up to 10 mmol/L Cr(VI) and completely reduce 1 mmol/L Cr(VI) to Cr(III) within 48 h. When the strain CTS-325 was induced with Cr(VI), a protein increased significantly in the whole cell proteins. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis revealed that this protein was a superoxide dismutase (SOD) homology. The measured superoxide dismutase activity was 2694 U/mg after three steps of purification. The SOD catalyzes the dismutation of the superoxide anion (O2*-) into hydrogen peroxide and molecular oxygen. This protein is considered to be one of the most important anti-oxidative enzymes for O. anthropi as it allows the bacterium to survive high oxygen stress environments, such as the environment produced during the reduction process of Cr(VI).
从中国铬酸盐厂分离到一株耐铬酸盐（VI）和还原的革兰氏阴性菌 CTS-325，根据其生化特性和 16S rDNA 序列分析，鉴定为食酸寡养单胞菌。该菌能耐受高达 10mmol/L 的 Cr(VI)，并能在 48 小时内将 1mmol/L 的 Cr(VI)完全还原为 Cr(III)。当菌株 CTS-325 受到 Cr(VI)诱导时，全细胞蛋白中一种蛋白质显著增加。液相色谱串联质谱（LC-MS/MS）分析表明，该蛋白是一种超氧化物歧化酶（SOD）同源物。经过三步纯化后，测得的超氧化物歧化酶活性为 2694U/mg。SOD 催化超氧阴离子（O2*-）歧化为过氧化氢和分子氧。这种蛋白质被认为是食酸寡养单胞菌最重要的抗氧化酶之一，因为它使细菌能够在高氧应激环境中存活，例如在 Cr(VI)还原过程中产生的环境中。
