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跌落冲击对苹果果实生理响应和基因表达的影响。

Influence of drop shock on physiological responses and genes expression of apple fruit.

机构信息

Experimental Teaching Center, Shenyang Normal University, Shenyang, Liaoning 110034, China.

College of Grain Science and Technology, Shenyang Normal University, Shenyang, Liaoning 110034, China.

出版信息

Food Chem. 2020 Jan 15;303:125424. doi: 10.1016/j.foodchem.2019.125424. Epub 2019 Aug 23.

DOI:10.1016/j.foodchem.2019.125424
PMID:31472384
Abstract

The aim of our study was to investigate the effect of drop shock on physiological responses and genes expression in harvested apple fruit stored at 20 ± 2 °C. Ethylene production, respiratory rate, firmness, soluble solid content, relative electrical conductivity, LOX (lipoxygenase) activity, MDA (malondialdehyde) content, variation of volatile compounds, ethylene biosynthetic genes, and ethylene receptor genes of apple fruit were examined. The results indicated that drop shock observably resulted in the increase of ethylene production, respiratory rate, soluble solid content, relative electrical conductivity, LOX activity, MDA content and gene expression levels in apples. Furthermore, drop shock significantly decreased firmness and high-intensitive drop shock stimulated the accumulation of aldehydes and esters in harvested apples. Overall, the greater impact on apple quality is the effect of larger amplitude of shock during truck transportation, which seriously reduced storage life and quality of postharvest apples.

摘要

本研究旨在探讨 20±2°C贮藏条件下,跌落冲击对采后苹果果实生理响应和基因表达的影响。检测了乙烯生成量、呼吸速率、硬度、可溶固形物含量、相对电导率、脂氧合酶(LOX)活性、丙二醛(MDA)含量、挥发性化合物变化、苹果果实乙烯生物合成基因和乙烯受体基因的表达。结果表明,跌落冲击显著增加了苹果的乙烯生成量、呼吸速率、可溶固形物含量、相对电导率、LOX 活性、MDA 含量和基因表达水平。此外,跌落冲击显著降低了苹果的硬度,高强度的跌落冲击刺激了采后苹果中醛类和酯类的积累。总的来说,在运输过程中卡车更大的振幅冲击对苹果品质的影响更大,严重降低了采后苹果的贮藏寿命和品质。

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