Osman Bilgen, Sagdilek Engin, Gümrükçü Merve, Göçenoğlu Sarıkaya Aslı
Bursa Uludag University, Department of Chemistry, Bursa, Turkey.
Bursa Uludag University, Department of Biophysics, Bursa, Turkey.
Colloids Surf B Biointerfaces. 2019 Nov 1;183:110456. doi: 10.1016/j.colsurfb.2019.110456. Epub 2019 Aug 23.
In this study, uric acid (UA)-imprinted poly (hydroxyethyl methacrylate-N-methacryloyl-amido-L-cysteine methyl ester)-Fe [poly(HEMA-MAC)-Fe] nanoparticle-embedded poly(acrylamide-methyl methacrylate) cryogel [p(AAm-MMA)-MIP] was synthesized for selective UA adsorption. The nanoparticles were prepared via molecular imprinting. The prepared p(AAm-MMA)-MIP cryogel was characterized by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and swelling test. The swelling degree of the p(AAm-MMA)-MIP cryogel was determined as to 7.56 g HO/g cryogel. The prepared cryogel was used for UA adsorption from aqueous solution.The effects of pH (4.0-8.0), initial UA concentration (5-40 mg/L), temperature (4 °C, 25 °C and 35 °C) and contact time on the UA adsorption capacity were detailedly investigated. UA adsorption data were applied to Langmuir and Freundlich isotherm models. The adsorption data were well fitted with pseudo-second order kinetic model. The thermodynamic parameters (ΔG ͦ, ΔH ͦ, ΔS) demonstrated that the adsorption process was endothermic and spotaneous at 4 °C, 25 °C and 35 °C. The cryogel was also used for UA adsorption from human serum. The effects of the composite cryogel treatment on blood cells and hemostatic parameters were evaluated by using hemogram analyses, coagulation studies, thromboelastography and platelet aggregation studies. The results showed that the cryogel treatment has an allowable effect on blood cell counts and hemostatic parameters demonstrating the applicability of prepared composite cryogel for UA removal from human serum.
在本研究中，合成了尿酸（UA）印迹的聚（甲基丙烯酸羟乙酯-N-甲基丙烯酰基-氨基-L-半胱氨酸甲酯）-铁[聚（HEMA-MAC）-Fe]纳米颗粒嵌入的聚（丙烯酰胺-甲基丙烯酸甲酯）冷冻凝胶[p（AAm-MMA）-MIP]，用于选择性吸附UA。通过分子印迹制备纳米颗粒。采用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和溶胀试验对制备的p（AAm-MMA）-MIP冷冻凝胶进行了表征。p（AAm-MMA）-MIP冷冻凝胶的溶胀度测定为7.56 g HO/g冷冻凝胶。制备的冷冻凝胶用于从水溶液中吸附UA。详细研究了pH值（4.0-8.0）、初始UA浓度（5-40 mg/L）、温度（4°C、25°C和35°C）和接触时间对UA吸附容量的影响。将UA吸附数据应用于Langmuir和Freundlich等温线模型。吸附数据与伪二级动力学模型拟合良好。热力学参数（ΔGͦ、ΔHͦ、ΔS）表明，在4°C、25°C和35°C下，吸附过程是吸热的且自发的。该冷冻凝胶还用于从人血清中吸附UA。通过血细胞分析、凝血研究、血栓弹性描记术和血小板聚集研究评估了复合冷冻凝胶处理对血细胞和止血参数的影响。结果表明，冷冻凝胶处理对血细胞计数和止血参数具有可接受的影响，证明了制备的复合冷冻凝胶用于从人血清中去除UA的适用性。
