School of Biotechnology, State Key Laboratory of Bioreactor Engineering, R&D Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China.
Shanghai Jiao Tong Univ, Sch Med, Int Peace Matern & Child Hlth Hosp, Dept Gynecol, Shanghai 200030, China.
Carbohydr Polym. 2019 Nov 15;224:115171. doi: 10.1016/j.carbpol.2019.115171. Epub 2019 Aug 9.
Gynecological cancers are the most commonly diagnosed forms of cancer among the female population. Chitooligosaccharides (COS)-hydrolysis products from chitosan-display high bioavailability, high water solubility, and low molecular weight properties. Here, we investigated the influence of COS on 11 gynecological tumor cell types, and subsequently elucidated molecular mechanisms through which the observed inhibition occurred. Initially, we used a controllable enzyme-membrane coupling reactor system to obtain COS with a high degree of polymerization; the yield of high-degree-polymerized COS (DP 5-12) obtained with this reactor system accounted for ∼75% yields (w/w). Using these COS materials, cell line assays showed that COS elicited the most significant anti-tumor activity against C33A cells, with anti-tumor mechanisms related to oxidative stress, as well as activation of intrinsic mitochondrial apoptosis and autophagic signaling. Thus, we provide experimental evidence to demonstrate how the enzyme-membrane coupling reactor system can generate COS that exert bioactivity against gynecological cancers.
妇科癌症是女性人群中最常见的癌症类型。壳寡糖（COS）-壳聚糖的水解产物具有生物利用度高、水溶性好、分子量低的特点。在这里，我们研究了 COS 对 11 种妇科肿瘤细胞类型的影响，并通过阐明观察到的抑制作用发生的分子机制来进一步研究。首先，我们使用可控酶-膜偶联反应器系统获得了具有高聚合度的 COS；该反应器系统获得的高聚合度 COS（DP5-12）的收率约为 75%（w/w）。使用这些 COS 材料，细胞系测定表明，COS 对 C33A 细胞表现出最强的抗肿瘤活性，抗肿瘤机制与氧化应激以及线粒体内在凋亡和自噬信号的激活有关。因此，我们提供了实验证据，证明了酶-膜偶联反应器系统如何产生对妇科癌症具有生物活性的 COS。
