水溶性壳聚糖-乳糖衍生物的物理化学、体外抗氧化和细胞毒性性质。
Physicochemical, in vitro antioxidant and cytotoxic properties of water-soluble chitosan-lactose derivatives.
机构信息
Centro de Investigaciones y Transferencia de Villa María (CIT-VM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Villa María (UNVM), Villa María, Argentina.
Instituto para el Desarrollo Agroindustrial y de la Salud (IDAS), CONICET-UNRC, Departamento de Química, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina.
出版信息
Carbohydr Polym. 2019 Nov 15;224:115158. doi: 10.1016/j.carbpol.2019.115158. Epub 2019 Aug 8.
In this study, water-soluble chitosan (Ch) derivatives were synthesized by the Maillard reaction between Ch and lactose. The Ch derivatives were characterized by FT-IR, H-NMR and SLS to determine their structure, degree of deacetylation (DD), and molecular weight (Mw). The solubility at physiological pH, the in vitro antioxidant activity against hydroxyl radical, anion superoxide radical and ABTS cation radical, and the cytotoxicity against epithelial cells of the rat ileum (IEC-18) were also evaluated. The Maillard reaction, derivatives with lower Mw and DD and greater solubility than Ch were obtained. The biological properties of the derivatives were dependent on the concentration, Mw and DD, with antioxidant activity greater than or equal to that of Ch and non-cytotoxic in a wide range of concentrations. The results indicate that Ch derivatization with lactose produces new water-soluble polysaccharides, with antioxidant activity and non-cytotoxic, which can be used as biomaterials for food and pharmaceutical applications.
在这项研究中，通过壳聚糖（Ch）与乳糖之间的美拉德反应合成了水溶性壳聚糖（Ch）衍生物。通过傅里叶变换红外光谱（FT-IR）、氢核磁共振（H-NMR）和小角激光散射（SLS）对 Ch 衍生物进行了表征，以确定其结构、脱乙酰度（DD）和分子量（Mw）。还评估了它们在生理 pH 下的溶解度、对羟基自由基、阴离子超氧自由基和 ABTS 阳离子自由基的体外抗氧化活性以及对大鼠回肠上皮细胞（IEC-18）的细胞毒性。美拉德反应产生了具有较低 Mw 和 DD 以及比 Ch 更高溶解度的衍生物。衍生物的生物学特性取决于浓度、Mw 和 DD，其抗氧化活性大于或等于 Ch，并且在广泛的浓度范围内无细胞毒性。结果表明，用乳糖对壳聚糖进行衍生化可产生具有抗氧化活性和非细胞毒性的新型水溶性多糖，可作为食品和药物应用的生物材料。
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