铁皮石斛多糖通过调节免疫和恢复环磷酰胺致免疫抑制模型小鼠肠道菌群失调发挥作用。
Dendrobium sonia polysaccharide regulates immunity and restores the dysbiosis of the gut microbiota of the cyclophosphamide-induced immunosuppressed mice.
机构信息
Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210004, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, China.
出版信息
Chin J Nat Med. 2019 Aug;17(8):600-607. doi: 10.1016/S1875-5364(19)30062-7.
To recognize the potential medicinal value of the Dendrobium sonia, polysaccharide (DSP) was extracted, purified, and investigated for its immunomodulatory activity. In vitro, DSP was shown to enhance the viability (MTT assay) and phagocytosis of macrophages. In cyclophosphamide-induced immunosuppressed mice, DSP increased serum levels of TNF-α, IL-6 and IFN-γ (enzyme-linked immunosorbent assay, ELISA), and ameliorated the imbalance of the community of gut microbiota as detected by 16S ribosomal RNA gene sequencing. These results suggest that DSP might be beneficial for patients under immunosuppressed conditions.
为了发掘铁皮石斛的潜在药用价值，本研究提取并纯化了铁皮石斛多糖（DSP），并对其免疫调节活性进行了研究。实验结果表明，铁皮石斛多糖（DSP）在体外可提高巨噬细胞的活力（MTT 法）和吞噬能力。在环磷酰胺诱导的免疫抑制小鼠模型中，DSP 可提高血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和干扰素-γ（IFN-γ）的水平（酶联免疫吸附试验，ELISA），并通过 16S 核糖体 RNA 基因测序改善肠道微生物群落失衡。这些结果表明，DSP 可能对免疫抑制患者有益。
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