Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, 230027, Hefei, China.
Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, 230027, Hefei, China.
Biochem Biophys Res Commun. 2019 Oct 22;518(4):678-684. doi: 10.1016/j.bbrc.2019.08.107. Epub 2019 Aug 28.
N-methyladenosine (mA) "readers" play an important role in mRNA functions and metabolism. YTHDC2, as one of the mA readers, controls fertileness through decreasing associated mRNA abundance and enhancing the translation efficiency of related mRNA via binding the targeted mA RNA. However, how YTH domain of YTHDC2 recognize mA RNA is still unknown. In this study, we determined the crystal structure of human YTHDC2 YTH domain, which adopts similar architecture to other solved YTH domain structures. YTHDC2 contains a conserved mA binding pocket, and similar RNA binding surface shared by YTHDC1.
N6-甲基腺苷（m6A）“读码器”在 mRNA 功能和代谢中发挥重要作用。YTH 结构域包含 2（YTHDC2）作为 m6A 读码器之一，通过结合靶向 m6A RNA 来降低相关 mRNA 的丰度并提高相关 mRNA 的翻译效率，从而控制生育能力。然而，YTHDC2 的 YTH 结构域如何识别 m6A RNA 仍然未知。在这项研究中，我们测定了人 YTHDC2 YTH 结构域的晶体结构，该结构采用与其他已解决的 YTH 结构域结构相似的架构。YTHDC2 包含一个保守的 m6A 结合口袋，以及 YTHDC1 共享的类似 RNA 结合表面。
