苦瓜多糖的提取、衍生化及抗氧化活性。
Extraction, derivatization and antioxidant activity of bitter gourd polysaccharide.
机构信息
Active Carbohydrate Research Institute, Chongqing Key Laboratory of Inorganic Functional Materials, College of Chemistry, Chongqing Normal University, Chongqing 401331, China.
Active Carbohydrate Research Institute, Chongqing Key Laboratory of Inorganic Functional Materials, College of Chemistry, Chongqing Normal University, Chongqing 401331, China.
出版信息
Int J Biol Macromol. 2019 Dec 1;141:14-20. doi: 10.1016/j.ijbiomac.2019.08.239. Epub 2019 Aug 29.
The effect of derivatization on the antioxidant activity of bitter gourd polysaccharide was investigated. Bitter gourd polysaccharide (P) was prepared with hot water. Carboxymethylated bitter gourd polysaccharide (CM-P) and acetylated bitter gourd polysaccharide (AcP) were successfully prepared, which were identified by IR and NMR spectra. On this basis, the antioxidant activities of three polysaccharides were tested, including hydroxyl radical scavenging capacity, DPPH radical scavenging capacity, and anti-lipid peroxidation capacity. The results showed that different chemical modifications had different enhancement on the antioxidant activities of bitter gourd polysaccharide. So, bitter gourd polysaccharide could be used to develop potential antioxidants.
研究了衍生化对苦瓜多糖抗氧化活性的影响。采用热水法制备苦瓜多糖（P），成功制备了羧甲基化苦瓜多糖（CM-P）和乙酰化苦瓜多糖（AcP），并通过 IR 和 NMR 光谱进行了鉴定。在此基础上，测试了三种多糖的抗氧化活性，包括羟基自由基清除能力、DPPH 自由基清除能力和抗脂质过氧化能力。结果表明，不同的化学修饰对苦瓜多糖的抗氧化活性有不同的增强作用。因此，苦瓜多糖可用于开发潜在的抗氧化剂。
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