School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China.
Qinghai Huzhu Barley Wine Co., Ltd., Haidong 810500, China.
Molecules. 2024 Feb 1;29(3):684. doi: 10.3390/molecules29030684.
In order to efficiently extract β-glucan from highland barley (HBG) and study its structural characterization and antioxidant activity, ultrasonic-microwave-assisted extraction (UME) was optimized by the response surface method (RSM). Under the optimal extraction conditions of 25.05 mL/g liquid-solid ratio, 20 min ultrasonic time, and 480 W microwave intensity, the DPPH radical scavenging activity of HBG reached 25.67%. Two polysaccharide fractions were purified from HBG, namely HBG-1 and HBG-2. Structural characterization indicated that HBG-1 and HBG-2 had similar functional groups, glycosidic linkages, and linear and complex chain conformation. HBG-1 was mainly composed of glucose (98.97%), while HBG-2 primarily consisted of arabinose (38.23%), galactose (22.01%), and xylose (31.60%). The molecular weight of HBG-1 was much smaller than that of HBG-2. Both HBG-1 and HBG-2 exhibited concentration-dependent antioxidant activity, and HBG-1 was more active. This study provided insights into the efficient extraction of HBG and further investigated the structure and antioxidant activities of purified components HBG-1 and HBG-2. Meanwhile, the results of this study imply that HBG has the potential to be an antioxidant in foods and cosmetics.
为了从青稞中高效提取β-葡聚糖，并研究其结构特征和抗氧化活性，采用响应面法（RSM）对超声-微波辅助提取（UME）进行了优化。在最佳提取条件下，即液固比为 25.05 mL/g、超声时间 20 min 和微波强度 480 W 下，青稞的 DPPH 自由基清除活性达到 25.67%。从青稞中纯化得到两种多糖组分，分别命名为 HBG-1 和 HBG-2。结构特征表明，HBG-1 和 HBG-2 具有相似的功能基团、糖苷键和线性及复杂链构象。HBG-1 主要由葡萄糖（98.97%）组成，而 HBG-2 主要由阿拉伯糖（38.23%）、半乳糖（22.01%）和木糖（31.60%）组成。HBG-1 的分子量明显小于 HBG-2。HBG-1 和 HBG-2 均表现出浓度依赖性的抗氧化活性，且 HBG-1 更为活跃。本研究为青稞的高效提取提供了思路，并进一步研究了纯化组分 HBG-1 和 HBG-2 的结构和抗氧化活性。同时，本研究结果表明，青稞具有成为食品和化妆品中抗氧化剂的潜力。
