Department of Respiratory Medicine, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, China,
Department of Respiratory Medicine, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, China.
Med Princ Pract. 2020;29(1):80-89. doi: 10.1159/000503020. Epub 2019 Sep 2.
Lung cancer is the main cause of cancer--related deaths worldwide, and the overall 5-year survival rate of non-small cell lung cancer (NSCLC) remained low. -MicroRNAs had been confirmed to be an important regulator in tumor progression, and they could serve as either tumor promoters or suppressors in NSCLC.
To identify the novel cancer-specific biomarkers for NSCLC patients, which may be useful to monitor tumor progression and improve NSCLC patients' survival.
The expression profile of miR-421 was analyzed in NSCLC samples using public datasets, including The Cancer Genome Atlas and GSE102286. The expression level of miR-421 was detected by reverse transcription-polymerase chain reaction. Cell proliferation and cell cycle were detected by Cell Counting Kit assay, flow cytometry assay, respectively. Kyoto Encyclopedia of Genes and Genomes analysis were applied to determine the biological roles of miR-421, based on the online DAVID system. Statistical comparisons between groups of normalized data were performed using t test or Mann-Whitney U test according to the test condition.
In this study, we focused on exploring the roles of miR-421 in NSCLC prognosis and growth. The present study for the first time showed that miR-421 was overexpressed in NSCLC and associated with a shorter overall survival time of patients with NSCLC. Bioinformatics analysis revealed miR-421 was involved in transcription, cell cycle, and insulin signaling pathway regulation. Furthermore, a gain of function assay showed that overexpression of miR-421 could promote NSCLC cell proliferation and cell cycle progression.
Our findings suggest that miR-421 might be a promising prognostic and therapeutic target for NSCLC.
肺癌是全球癌症相关死亡的主要原因，而非小细胞肺癌（NSCLC）的总体 5 年生存率仍然较低。 - microRNAs 已被证实是肿瘤进展的重要调节因子，它们在 NSCLC 中既可以作为肿瘤促进因子，也可以作为肿瘤抑制因子。
确定 NSCLC 患者的新型癌症特异性生物标志物，这些标志物可能有助于监测肿瘤进展并改善 NSCLC 患者的生存率。
使用公共数据集（包括癌症基因组图谱和 GSE102286）分析 NSCLC 样本中的 miR-421 表达谱。通过逆转录聚合酶链反应检测 miR-421 的表达水平。通过细胞计数试剂盒测定法、流式细胞术分别检测细胞增殖和细胞周期。根据在线 DAVID 系统，应用京都基因与基因组百科全书分析来确定 miR-421 的生物学作用。使用 t 检验或曼-惠特尼 U 检验根据检验条件对组间归一化数据进行统计比较。
在这项研究中，我们专注于探索 miR-421 在 NSCLC 预后和生长中的作用。本研究首次表明，miR-421 在 NSCLC 中过表达，与 NSCLC 患者的总生存时间较短相关。生物信息学分析显示，miR-421 参与转录、细胞周期和胰岛素信号通路调节。此外，功能获得实验表明，miR-421 的过表达可以促进 NSCLC 细胞增殖和细胞周期进程。
我们的研究结果表明，miR-421 可能是 NSCLC 有前途的预后和治疗靶点。
