Key Laboratory of Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
School of Pharmacy, Yantai University, Yantai, 264005, China.
Appl Microbiol Biotechnol. 2019 Oct;103(20):8559-8569. doi: 10.1007/s00253-019-10018-7. Epub 2019 Aug 31.
Phycocyanin (PC) is a light-harvesting protein isolated from Spirulina and has health benefits for a range of diseases including pulmonary fibrosis (PF). In this study, a bleomycin-induced pulmonary fibrosis model was used to determine whether PC attenuates PF and modulates the intestinal microbiota. The results showed that PC intervention attenuated the pulmonary fibrosis, demonstrated by hematoxylin-eosin staining (HE), Masson's trichrome staining, and lung dry-wet weight ratio, and PC significantly inhibited the production of interleukin-1 beta (IL-1β), tumor necrosis factor-α (TNF-α), and lipopolysaccharide (LPS). Additionally, intestinal microbiota analysis revealed that PC intervention significantly increased the bacterial diversity and richness. Correlation analysis indicated that 9 families and 17 genes were significantly associated with at least 1 physiological index. And PC intervention significantly decreased the bacteria which is related to inflammation and dramatically increased the SCFAs-producing bacteria and probiotics. These data indicated that PC can decrease the pro-inflammatory cytokines and regulate the intestinal microbiota in BLM-induced PF mice.
藻蓝蛋白（PC）是一种从螺旋藻中分离出的光捕获蛋白，对一系列疾病（包括肺纤维化（PF））都有健康益处。在这项研究中，使用博来霉素诱导的肺纤维化模型来确定 PC 是否能减轻 PF 并调节肠道微生物群。结果表明，PC 干预减轻了肺纤维化，通过苏木精-伊红染色（HE）、马松三色染色和肺干湿重比来证明，PC 显著抑制白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和脂多糖（LPS）的产生。此外，肠道微生物组分析显示，PC 干预显著增加了细菌多样性和丰富度。相关性分析表明，9 个科和 17 个基因与至少 1 个生理指标显著相关。PC 干预显著降低了与炎症相关的细菌，显著增加了 SCFAs 产生菌和益生菌。这些数据表明，PC 可以减少促炎细胞因子并调节 BLM 诱导的 PF 小鼠的肠道微生物群。
