UNESP - São Paulo State University, School of Veterinary Medicine and Animal Science, Botucatu, Brasil.
UNESP - São Paulo State University, Institute of Biosciences of Botucatu, Botucatu, Brasil.
Biol Trace Elem Res. 2020 Jun;195(2):648-657. doi: 10.1007/s12011-019-01863-8. Epub 2019 Aug 31.
This study aimed to evaluate the quality of the royal jelly produced by Apis mellifera bees in the presence of different iron concentrations (ferrous sulfate heptahydrate-0, 25, 50, and 100 mg L). Two-dimensional electrophoresis was used for the fractionation of royal jelly proteins, and iron level was quantified using flame atomic absorption spectrometry technique. The proteins were identified using electrospray ionisation mass spectrometry. Analysis of variance followed by the Tukey test (P < 0.05) was utilised. Dietary supplementation with mineral Fe affected the protein content and number of proteins in the experimental period. Further, the diet containing the highest iron concentration showed a greater number of spots containing iron, as well as in the abdomen of the bees. The most protein containing Fe were classified as major royal jelly proteins. These results showed that Fe influenced the quality of royal jelly and can improve its nutritional value.
本研究旨在评估不同铁浓度（硫酸亚铁七水合物-0、25、50 和 100 mg/L）存在下，蜜蜂产生的蜂王浆的质量。使用二维电泳对蜂王浆蛋白进行分离，并使用火焰原子吸收光谱技术定量铁含量。使用电喷雾电离质谱对蛋白质进行鉴定。采用方差分析和 Tukey 检验（P < 0.05）。膳食补充矿物质 Fe 会影响实验期间的蛋白质含量和蛋白质数量。此外，含有最高铁浓度的饮食显示出更多含有铁的斑点，以及蜜蜂的腹部。含有最多 Fe 的蛋白质被归类为主要蜂王浆蛋白。这些结果表明，Fe 影响蜂王浆的质量，并可以提高其营养价值。
