翅斑和彗星试验作为检测果蝇基因毒性的有用分析方法。
The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila.
作者信息
Marcos Ricard, Carmona Erico R
机构信息
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Barcelona, Spain.
Núcleo de Investigación en Bioproductos y Materiales Avanzados (BioMA), Facultad de Ingeniería, Universidad Católica de Temuco, Temuco, Chile.
出版信息
Methods Mol Biol. 2019;2031:337-348. doi: 10.1007/978-1-4939-9646-9_19.
In spite of its pioneer use in detecting mutational processes, Drosophila still plays an important role in those studies aiming to detect and quantify the induction of DNA damage. Here we describe two assays, one detecting primary damage (the Comet assay) and the other detecting somatic mutation and recombination effects (wing-spot test). It is important to emphasize that somatic recombination is a key event in cancer development and no assays exist at present to detect and quantify somatic recombination processes, other than the spot tests developed in Drosophila.
尽管果蝇在检测突变过程中率先得到应用，但在旨在检测和量化DNA损伤诱导的研究中，它仍然发挥着重要作用。在此，我们描述两种检测方法，一种检测原发性损伤（彗星试验），另一种检测体细胞突变和重组效应（翅斑试验）。必须强调的是，体细胞重组是癌症发展中的关键事件，除了在果蝇中开发的斑点试验外，目前不存在检测和量化体细胞重组过程的方法。
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