二氧化钛纳米颗粒：黑腹果蝇中的毒性和遗传毒性（神经母细胞中的 SMART 眼点试验和彗星试验）
Titanium dioxide nanoparticles: Toxicity and genotoxicity in Drosophila melanogaster (SMART eye-spot test and comet assay in neuroblasts).
作者信息
Sario Sara, Silva Amélia M, Gaivão Isabel
机构信息
Department of Genetics and Biotechnology (DGB - ECVA), University of Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, P-5001-801 Vila Real, Portugal; Department of Biology and Environment (DeBA - ECVA), University of Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, P-5001-801 Vila Real, Portugal.
Department of Biology and Environment (DeBA - ECVA), University of Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, P-5001-801 Vila Real, Portugal; Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB-UTAD), P-5001-801 Vila Real, Portugal.
出版信息
Mutat Res Genet Toxicol Environ Mutagen. 2018 Jul;831:19-23. doi: 10.1016/j.mrgentox.2018.05.001. Epub 2018 May 4.
Titanium dioxide nanoparticles (TiONP) are used in the food, drug, and cosmetics industries and evaluation of their human and environmental toxicity is required. We have tested the toxicity of TiONP (anatase) with respect to developmental effects and DNA damage in Drosophila melanogaster strain Ok, using the eye-spot Somatic Mutation and Recombination Test (SMART) and the comet assay (neuroblasts). For the survival assay, TiONP were supplied to adult flies for 72 h and no adverse effects were seen. TiONP were supplied chronically for the prolificacy, SMART, and comet assays. TiONP increased fly prolificacy. With regard to genotoxicity, an increase was observed in the eye-spot SMART assay at 8 μg/mL dose, but not in the neuroblast comet assay for DNA damage.
二氧化钛纳米颗粒（TiONP）被用于食品、药品和化妆品行业，因此需要评估其对人类和环境的毒性。我们使用眼斑体细胞突变和重组试验（SMART）以及彗星试验（神经母细胞），测试了锐钛矿型TiONP对黑腹果蝇Ok品系发育效应和DNA损伤的毒性。在生存试验中，将TiONP提供给成年果蝇72小时，未观察到不良影响。在繁殖力、SMART试验和彗星试验中，长期提供TiONP。TiONP提高了果蝇的繁殖力。关于遗传毒性，在8μg/mL剂量的眼斑SMART试验中观察到增加，但在DNA损伤的神经母细胞彗星试验中未观察到增加。
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