Amagasa M, Mizoi K, Yoshimoto T, Suzuki J
Division of Neurosurgery, University School of Medicine, Sendai, Japan.
No Shinkei Geka. 1988 Nov;16(12):1363-71.
A study was made of protective effect of several drugs (mannitol, phenytoin, vitamin E and dexamethasone) against the deprivation of both glucose and oxygen from the bathing medium on electrical activities of guinea-pig hippocampal neurons in vitro. Using guinea-pig hippocampal slice, we recorded antidromic field potentials in the granular cell layer of the dentate gyrus with stimulating mossy fibers. "Ischemia" of a slice was achieved by substracting both glucose and oxygen from the perfusing medium. In standard medium, after 10 minutes of ischemia, field potentials had minimum recovery with an amplitude of 5% of control after 60 minutes in the standard medium. Mannitol treatment had no protective effect, but phenytoin, vitamin E and dexamethasone had clear dose-dependent effect. The protective effect was evaluated by recovery of field potential amplitude of the 60 minutes postischemic response and histological examination of the brain slice tissue. The degree of the histological damage was correlated with recovery of field potential. In this experiment we have demonstrated that phenytoin and vitamin E obviously have the protective action against ischemic neuronal damage in the guinea-pig hippocampal slice and the combined application of these drugs were more effective.
研究了几种药物（甘露醇、苯妥英、维生素E和地塞米松）对体外培养的豚鼠海马神经元电活动免受灌流液中葡萄糖和氧气剥夺的保护作用。利用豚鼠海马脑片，通过刺激苔藓纤维，在齿状回颗粒细胞层记录逆向场电位。通过从灌流液中去除葡萄糖和氧气来实现脑片的“缺血”。在标准培养基中，缺血10分钟后，场电位恢复极小，在标准培养基中60分钟后振幅仅为对照的5%。甘露醇处理无保护作用，但苯妥英、维生素E和地塞米松有明显的剂量依赖性效应。通过缺血后60分钟场电位振幅的恢复以及脑片组织的组织学检查来评估保护作用。组织学损伤程度与场电位恢复相关。在本实验中，我们证明苯妥英和维生素E对豚鼠海马脑片缺血性神经元损伤有明显保护作用，且这些药物联合应用更有效。
