Fujimoto S, Mizoi K, Oba M, Suzuki J
No Shinkei Geka. 1984 Feb;12(2):171-80.
We have previously reported that the combined administration of mannitol and perfluorochemical blood substitutes is evidently effective in protecting the brain from cerebral ischemia. This experimental study was designed to develop more effective method in suppressing brain infarction than the combined treatment of mannitol and PFC. Using the "Canine model of complete ischemic brain regulated with a perfusion method" in which it is possible to control the degree of blood flow to a cerebral hemisphere via a perfusion pump, the effect of eight agents including six kinds as the free radical scavenger on cerebral ischemia was investigated. Eight agents were mannitol, vitamin E (Vit. E), dimethyl sulfoxide (DMSO), vitamin C, glycerol, nizofenone (Y-9179), dexamethasone (Dexa.) and suloctidil (MY-103). After pretreatment with each agent, blood flow was reduced via the pump to 1/10 of the normal state and 1 hour later, return to the normal state was allowed. Subsequent changes in EEG were observed and the effects of the drugs evaluated. In the control group, no recovery of electrical activity was seen, but in six groups among eight treated groups, i.e., treated with mannitol, Vit. E, DMSO, MY-103, Y-9179 and Dexa, gradual emergence of slow waves was observed. And more favorable effects were found when the combined administration of mannitol, Vit. E and Dexa was made in the same experimental schedule as compared with the single administration of each of these drugs. Furthermore in the animals administered with PFC in combination with mannitol, Vit. E and Dexa, flattening of electrical activity could not be seen throughout the period of severe ischemia. Moreover, the power of electrical activity recovered nearly to the preischemic state immediately after recirculation. Although the possible mechanisms are not yet completely clarified, the present results are thought to indicate that this new combination therapy utilizing PFC with mannitol, Vit. E and Dexa may be useful in the treatment of cerebral ischemia.
我们之前曾报道，甘露醇与全氟化合物血液代用品联合给药在保护大脑免受脑缺血损伤方面具有显著效果。本实验研究旨在开发一种比甘露醇与全氟化合物联合治疗更有效的抑制脑梗死的方法。使用“通过灌注方法调节的完全缺血性脑犬模型”，该模型能够通过灌注泵控制大脑半球的血流程度，研究了包括六种自由基清除剂在内的八种药物对脑缺血的影响。八种药物分别为甘露醇、维生素E（Vit.E）、二甲基亚砜（DMSO）、维生素C、甘油、尼唑苯酮（Y - 9179）、地塞米松（Dexa.）和舒洛地尔（MY - 103）。在每种药物预处理后，通过泵将血流减少至正常状态的1/10，1小时后，恢复至正常状态。随后观察脑电图的变化并评估药物的效果。在对照组中，未见电活动恢复，但在八个治疗组中的六个组，即使用甘露醇、Vit.E、DMSO、MY - 103、Y - 9179和Dexa治疗的组中，观察到慢波逐渐出现。并且与单独使用这些药物中的每一种相比，在相同的实验方案中联合使用甘露醇、Vit.E和Dexa时发现了更有利的效果。此外，在联合使用全氟化合物与甘露醇、Vit.E和Dexa的动物中，在严重缺血期间整个阶段均未见电活动变平。而且，再灌注后电活动的功率立即几乎恢复到缺血前状态。尽管可能的机制尚未完全阐明，但目前的结果被认为表明这种利用全氟化合物与甘露醇、Vit.E和Dexa的新联合疗法可能对脑缺血的治疗有用。
