[2017年河南省分离的7株麻疹病毒血凝素基因特征分析]

[Analysis on the hemagglutinin genetic characteristic of 7 measles virus isolated in Henan Province in 2017].

作者信息

Feng D X, Zhang L, Lyu Y Y, Yang J H, Li G W, Xu J, Zhang Y Y

机构信息

Institute of Expanded Programme on Immunization, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2019 Sep 6;53(9):929-933. doi: 10.3760/cma.j.issn.0253-9624.2019.09.012.

DOI:10.3760/cma.j.issn.0253-9624.2019.09.012

PMID:31474076

Abstract

Analyze the genetic characteristic of Hemagglutinin(H) gene of measles viruses isolated in Henan Province in 2017. Swab samples collected from 7 lab confirmed measles cases, and we got the measles virus by Vero/Slam inoculation. Fragment of H genes were amplified by reverse transcription polymerase chain reaction(RT-PCR), then the PCR products were sequenced and analyzed. The age of the 7 measles confirmed cases were between 1 and 50 years old, and all of them were males. All the 7 measles viruses were identified as H1a genotype, and the average distance of the nucleotides and the amino acids was 0.005, respectively. Compared with the Shanghai-191/China-vaccine, there were some changes in isolated virus, such as 240(th), 397(th) and 381(st) sites in the amino acid sequence. The measles genotype which isolated in Henan Province in 2017 was H1a. There were some difference from Shanghai-191/China-vaccine in the nucleotides sequence of H gene, which suggested that it's necessary to strengthen the monitor the variation of measles virus.

摘要

分析2017年河南省分离的麻疹病毒血凝素（H）基因的遗传特征。从7例实验室确诊的麻疹病例中采集咽拭子样本，通过Vero/Slam接种获得麻疹病毒。采用逆转录聚合酶链反应（RT-PCR）扩增H基因片段，然后对PCR产物进行测序和分析。7例确诊麻疹病例的年龄在1至50岁之间，均为男性。7株麻疹病毒均鉴定为H1a基因型，核苷酸和氨基酸的平均距离分别为0.005。与上海-191/中国疫苗株相比，分离出的病毒在氨基酸序列的第240、397和381位点等有一些变化。2017年在河南省分离的麻疹基因型为H1a。其H基因核苷酸序列与上海-191/中国疫苗株存在一些差异，提示有必要加强对麻疹病毒变异的监测。