Department of Chemistry, Wayne State University, Detroit, MI 48202, United States.
J Chromatogr A. 2019 Oct 25;1604:460482. doi: 10.1016/j.chroma.2019.460482. Epub 2019 Aug 23.
The solvation parameter model is used to characterize the separation properties of 52 fused-silica, wall-coated, open-tubular columns at 5 evenly-spaced intervals over the temperature range 60 to 140 °C. Statistical and principal component factor analysis are used to identify stationary phases of similar separation characteristics considered near selectivity equivalent and stationary phases with specific differences in selectivity for method development. The column database includes examples of all common stationary phase chemistries and manufacturing processes (surface preparation, deactivation, surface bonding, and immobilization). The influence of monomer composition on the separation properties of poly(siloxane) and poly(ethylene glycol) stationary phases is fully characterized and used to demonstrate where gaps in the selectivity space exist in the column database. Interfacial adsorption was observed as an important retention mechanism for biscyanopropylsiloxane-containing stationary phases for n-alkanes but for other compounds and stationary phases gas-liquid partition appears to be the dominant retention mechanism. There is poor agreement between McReynolds phase constants and the system constants of the solvation parameter model for the characterization of stationary phase selectivity.
该溶离参数模型被用于描述 52 根键合于熔融石英、管壁涂布、开管柱在温度范围 60 至 140°C 时，以 5 个等间距间隔下的分离特性。统计与主成分因子分析被用于鉴别具有相似选择性的固定相，视为选择性相当，以及具有特定选择性差异的固定相，可用于方法开发。该柱数据库包含了所有常见固定相化学与制造程序的范例（表面准备、去活化、表面键合、与固定化）。单体组成对于聚（硅氧烷）与聚（乙二醇）固定相的分离特性的影响被全面地描绘，并被用于展示在该柱数据库的选择性空间中存在的缺口。界面吸附被观察为含双氰丙基硅氧烷固定相对于正烷烃的重要保留机制，但对于其他化合物与固定相，气-液分配似乎为主要的保留机制。对于固定相选择性的描述，McReynolds 相常数与溶离参数模型的系统常数之间的一致性很差。
