Zhang Yurong, Zhang Mengdi, Yu Jie, Zhu Wei, Wang Qiaoyun, Pan Xiaohong, Gao Xue, Yang Jing, Sun Hongliu
School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, China.
Shandong Academy of Medical Sciences, Jinan, China.
Front Cell Neurosci. 2019 Aug 14;13:375. doi: 10.3389/fncel.2019.00375. eCollection 2019.
Previous studies have reported the possible neuroprotective effects of xenon treatment. The purpose of this study was to define the range of effective xenon ratio, most effective xenon ratio, and time-window for intervention in the kainic acid (KA) - induced status epilepticus (SE) rat model. Different ratios of xenon (35% xenon, 21% oxygen, 44% nitrogen, 50% xenon, 21% oxygen, 29% nitrogen, 70% xenon, 21% oxygen, and 9% nitrogen) were used to treat the KA-induced SE. Our results confirmed the anti-seizure role of 50 and 70% xenon mixture, with a stronger effect from the latter. Further, 70% xenon mixture was dispensed at three time points (0 min, 15 min delayed, and 30 min delayed) after KA administration, and the results indicated the anti-seizure effect at all treated time points. The results also established that the neuronal injury in the hippocampus and entorhinal cortex (EC), assessed using Fluoro-Jade B (FJB) staining, were reversed by the xenon inhalation, and within 30 min after KA administration. Our study, therefore, indicates the appropriate effective xenon ratio and time-window for intervention that can depress seizures. The prevention of neuronal injury and further reversal of the loss of effective control of depress network in the hippocampus and EC may be the mechanisms underlying the anti-seizure effect of xenon.
先前的研究报道了氙气治疗可能具有的神经保护作用。本研究的目的是确定在 kainic 酸（KA）诱导的癫痫持续状态（SE）大鼠模型中，有效氙气比例范围、最有效氙气比例以及干预的时间窗。使用不同比例的氙气（35%氙气、21%氧气、44%氮气；50%氙气、21%氧气、29%氮气；70%氙气、21%氧气、9%氮气）治疗 KA 诱导的 SE。我们的结果证实了 50%和 70%氙气混合物具有抗癫痫作用，且后者效果更强。此外，在 KA 给药后的三个时间点（0 分钟、延迟 15 分钟、延迟 30 分钟）给予 70%氙气混合物，结果表明在所有治疗时间点均有抗癫痫作用。结果还表明，使用 Fluoro-Jade B（FJB）染色评估，海马体和内嗅皮质（EC）中的神经元损伤通过氙气吸入得以逆转，且发生在 KA 给药后 30 分钟内。因此，我们的研究表明了可抑制癫痫发作的合适有效氙气比例和干预时间窗。预防神经元损伤以及进一步逆转海马体和 EC 中抑制网络有效控制的丧失可能是氙气抗癫痫作用的潜在机制。
