School of Electronic and Electrical Engineering, Wuhan Textile University, Wuhan, 430200, People's Republic of China.
Hubei Province Engineering Research Center for Intelligent Micro-nano Medical Equipment and Key Technologies, Wuhan, 30200, People's Republic of China.
Biomed Microdevices. 2019 Sep 2;21(4):87. doi: 10.1007/s10544-019-0434-5.
As we all know, microvalve holds great importance for microfluidic manipulation in chip. Herein, a simple method of high-performance multiplex microvalves chip fabrication was reported. In this method, a sandwich structure is established by inserting a polydimethylsiloxane (PDMS) membrane into two glasses, which is cheap and simple without any complex silicon-based device or soft lithography. Taking advantages of both the elasticity of the PDMS and the rigidity of glass, the microvalve chip showed good controls performance and had the ability of multiplex integration. Moreover, aided by a computer design program, this microvalves chip can be performed automatically, showing great potential to develop new highly integrated microfluidic devices. In addition, the fabricated multiplex microvalve chip is further successfully used for staining tumor cells automatically, improving the efficiency of cell identification process and reducing human errors. These results indicate this method opens up new avenues for multiplex microvalves fabrication and its biological application.
众所周知，微阀对于芯片上的微流控操作至关重要。在此，我们报道了一种用于制造高性能多路复用微阀芯片的简单方法。在这种方法中，通过将聚二甲基硅氧烷（PDMS）膜插入两块玻璃之间来构建三明治结构，这种方法便宜且简单，无需任何复杂的硅基器件或软光刻技术。利用 PDMS 的弹性和玻璃的刚性，微阀芯片表现出良好的控制性能，并且具有多路复用集成的能力。此外，借助计算机设计程序，这种微阀芯片可以自动执行，为开发新型高度集成的微流控器件提供了巨大的潜力。此外，所制造的多路复用微阀芯片还进一步成功地用于自动染色肿瘤细胞，提高了细胞鉴定过程的效率并减少了人为错误。这些结果表明，这种方法为多路复用微阀的制造及其生物应用开辟了新途径。
