氨基酸功能化多壁碳纳米管 (MWCNTs-NH) 对序批式反应器性能、酶活性和微生物群落的长期影响的研究进展。
Insights into long-term effects of amino-functionalized multi-walled carbon nanotubes (MWCNTs-NH) on the performance, enzymatic activity and microbial community of sequencing batch reactor.
机构信息
Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Qingdao 266100, China.
Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
出版信息
Environ Pollut. 2019 Nov;254(Pt B):113118. doi: 10.1016/j.envpol.2019.113118. Epub 2019 Aug 27.
Carbon nanotubes (CNTs) inevitably enter domestic sewage and industrial wastewater with the continuous increase of their production and application field. The potential effect of CNTs on biological wastewater treatment processes has raised wide concerns due to their biotoxicity. In the present study, the performance, microbial community and enzymatic activity of sequencing batch reactors (SBRs) were evaluated under 148-day exposure of amino-functionalized multi-walled CNTs (MWCNTs-NH) at 10 and 30 mg/L. The COD removal efficiency at 10 and 30 mg/L MWCNTs-NH gradually reduced from 91.03% and 90.43% on day to 89.11% and 86.70% on day 148, respectively. The NH-N removal efficiency at 10 and 30 mg/L MWCNTs-NH gradually reduced from 98.98% and 98.46% on day 1 to 96.65% and 63.39% on day 148, respectively. Compared to 0 mg/L MWCNTs-NH, the oxygen-utilizing rate, ammonia-oxidizing rate, nitrite-oxidizing rate, nitrite-reducing rate and nitrate-reducing rate at 30 mg/L MWCNTs-NH were decreased by 52.35%, 60.58%, 55.12%, 56.56% and 57.42% on day 148, respectively. The microbial reactive oxygen species and lactate dehydrogenase release on day 148 was increased by 59.71% and 55.28% at 30 mg/L MWCNTs-NH, respectively. The key microbial enzymatic activity related to nitrogen removal decreased with the increase of operation time under MWCNTs-NH stress. The relative abundances of Nitrosomonas, Nitrosospira, Nitrospira and some denitrifying bacteria at 10 mg/L MWCNTs-NH gradually reduced with an increment in operation time. The changes of nitrogen removal rate, microbial community and enzymatic activity of SBR were related to the time-cumulative nonlinear inhibition effect under long-term exposure.
碳纳米管（CNTs）在其生产和应用领域的不断增加，不可避免地会进入生活污水和工业废水中。由于其生物毒性，CNTs 对生物废水处理过程的潜在影响引起了广泛关注。在本研究中，在 148 天的时间里，评估了氨基酸功能化多壁 CNTs（MWCNTs-NH）在 10 和 30 mg/L 下对序列间歇式反应器（SBR）的性能、微生物群落和酶活性的影响。在 10 和 30 mg/L MWCNTs-NH 下，COD 去除效率分别从第 1 天的 91.03%和 90.43%逐渐降低到第 148 天的 89.11%和 86.70%。在 10 和 30 mg/L MWCNTs-NH 下，NH-N 去除效率分别从第 1 天的 98.98%和 98.46%逐渐降低到第 148 天的 96.65%和 63.39%。与 0 mg/L MWCNTs-NH 相比，在 30 mg/L MWCNTs-NH 下，第 148 天的耗氧率、氨氧化率、亚硝酸盐氧化率、亚硝酸盐还原率和硝酸盐还原率分别降低了 52.35%、60.58%、55.12%、56.56%和 57.42%。在 30 mg/L MWCNTs-NH 下，第 148 天微生物活性氧和乳酸脱氢酶的释放分别增加了 59.71%和 55.28%。随着 MWCNTs-NH 胁迫下运行时间的增加，与氮去除相关的关键微生物酶活性降低。在 10 mg/L MWCNTs-NH 下，硝化菌、亚硝化菌、硝化螺旋菌和一些反硝化菌的相对丰度随着运行时间的增加逐渐降低。SBR 的脱氮率、微生物群落和酶活性的变化与长期暴露下的时间累积非线性抑制作用有关。
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