Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Jiangsu, 225009, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, 225009, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of A griculture of China, Yangzhou University, Yangzhou, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou University, Jiangsu, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Jiangsu, 225009, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, 225009, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of A griculture of China, Yangzhou University, Yangzhou, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou University, Jiangsu, China.
Int J Med Microbiol. 2019 Dec;309(8):151337. doi: 10.1016/j.ijmm.2019.151337. Epub 2019 Aug 12.
Salmonella Enteritidis (SE) is a highly adapted pathogen causing severe economic losses in the poultry industry worldwide. Chickens infected by SE are a major source of human food poisoning. Vaccination is an effective approach to control SE infections. This study evaluated the immunogenicity and protective efficacy of a SE sptP deletion mutant (C50336ΔsptP) as a live attenuated vaccine (LAV) candidate in chickens. 14 day-old specific pathogen-free (SPF) chickens were intramuscularly immunized with various doses of C50336ΔsptP. Several groups of chickens were challenged with the virulent wild-type SE strain Z-11 via the same route at 14 days post vaccination. Compared to the control group, the groups vaccinated with 1 × 10, 1 × 10 and 1 × 10 colony-forming units (CFU) of C50336ΔsptP exhibited no clinical symptoms after immunization. Only slight pathological changes occurred in the organs of the 1 × 10 CFU vaccinated group. C50336ΔsptP bacteria were cleared from the organs of immunized chickens within 14 days after vaccination. Lymphocyte proliferation and serum cytokine analyses indicated that significant cellular immune responses were induced after the vaccination of C50336ΔsptP. Compared to the control group, specific IgG antibody levels increased significantly in vaccinated chickens, and the levels increased markedly after the challenge. The 1 × 10, 1 × 10, and 1 × 10 CFU vaccinated chickens groups showed no clinical symptoms or pathological changes, and no death after the lethal challenge. Whereas severe clinical signs of disease and pathological changes were observed in the control group chickens after the challenge. These results suggest that a single dose of C50336ΔsptP could be an effective LAV candidate to against SE infection in chickens.
肠炎沙门氏菌（SE）是一种高度适应的病原体，在全球范围内给家禽业造成严重的经济损失。感染 SE 的鸡是人类食物中毒的主要来源。疫苗接种是控制 SE 感染的有效方法。本研究评估了 SE sptP 缺失突变体（C50336ΔsptP）作为活减毒疫苗（LAV）候选物在鸡中的免疫原性和保护效力。14 日龄无特定病原体（SPF）鸡通过肌肉注射不同剂量的 C50336ΔsptP 进行免疫。在接种后 14 天，通过相同途径用强毒野生型 SE 菌株 Z-11 对几组鸡进行攻毒。与对照组相比，接种 1×10、1×10 和 1×10 个集落形成单位（CFU）的 C50336ΔsptP 组在免疫后没有出现临床症状。仅接种 1×10 CFU 组的器官出现轻微的病理变化。C50336ΔsptP 细菌在接种后 14 天内从免疫鸡的器官中清除。淋巴细胞增殖和血清细胞因子分析表明，接种 C50336ΔsptP 后诱导了显著的细胞免疫反应。与对照组相比，接种鸡的特异性 IgG 抗体水平显著升高，攻毒后显著升高。1×10、1×10 和 1×10 CFU 接种组在致死性攻毒后无临床症状或病理变化，也无死亡。而对照组鸡在攻毒后出现严重的疾病临床症状和病理变化。这些结果表明，单次剂量的 C50336ΔsptP 可能是一种有效的 LAV 候选物，可用于预防鸡感染 SE。
