Yang Jen-Jia, Cheng Aristine, Tai Hui-Ming, Chang Li-Wen, Hsu Ming-Chu, Sheng Wang-Huei
Department of Internal Medicine, Po Jen General Hospital, Taipei, Taiwan.
Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Microb Drug Resist. 2020 Feb;26(2):110-117. doi: 10.1089/mdr.2019.0048. Epub 2019 Aug 30.
Nemonoxacin is a nonfluorinated quinolone with good bactericidal effects against quinolone-resistant Gram-positive microorganisms. The inducible resistance of nemonoxacin against clinically relevant Gram-positive pathogens was compared with ciprofloxacin, levofloxacin, and moxifloxacin. Three strains of each bacterial species, including , , , and , were cultured. All clinical isolates had wild-type , and , before further test. DNA sequencing for the quinolone resistance determination region (QRDR) of gyrase and topoisomerase genes was performed. Nemonoxacin had the lowest minimum inhibitory concentrations (MICs) among all quinolones. During exposure to nemonoxacin, the MIC values did not increase for , , and , and revealed fourfold increase of over three cycles of a stepwise resistance selection. DNA sequencing did not show inducible QRDR resistance of nemonoxacin group. Compared to other fluoroquinolones, nemonoxacin has a low potential for inducing resistant pathogens.
奈诺沙星是一种对喹诺酮耐药革兰氏阳性微生物具有良好杀菌作用的非氟喹诺酮类药物。将奈诺沙星对临床相关革兰氏阳性病原体的诱导耐药性与环丙沙星、左氧氟沙星和莫西沙星进行了比较。培养了每种细菌的三株菌株，包括金黄色葡萄球菌、肺炎链球菌、粪肠球菌和屎肠球菌。在进一步进行试验之前，所有临床分离株的gyrA、parC和parE均为野生型。对gyrase和topoisomerase基因的喹诺酮耐药决定区（QRDR）进行了DNA测序。奈诺沙星在所有喹诺酮类药物中具有最低的最低抑菌浓度（MIC）。在接触奈诺沙星期间，金黄色葡萄球菌、肺炎链球菌和粪肠球菌的MIC值没有增加，并且在逐步耐药选择的三个周期中屎肠球菌的MIC值显示增加了四倍。DNA测序未显示奈诺沙星组的诱导型QRDR耐药性。与其他氟喹诺酮类药物相比，奈诺沙星诱导耐药病原体的可能性较低。
