College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, PR China.
College of Electrical and Information, Heilongjiang Bayi Agricultural University, Daqing, PR China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2019 Nov;36(11):1677-1687. doi: 10.1080/19440049.2019.1657966. Epub 2019 Sep 3.
An extraction method based on metal-organic framework has been developed and applied to acetanilide herbicides, including metazachlor, propanil, pretilachlor, and butachlor, in black beans, red beans, and kidney beans. The acetanilide herbicides are extracted with a mixture of ethyl acetate and n-hexane. The extract solution is absorbed and purified with metal-organic framework MIL-101 (Zn). The separation and determination of four acetanilide herbicides were implemented by high-performance liquid chromatography. The experimental parameters were evaluated by a univariate method and orthogonal experiments. The presented method can obtain effective extraction and purification. The detection limits for metazachlor, propanil, pretilachlor, and butachlor were 0.58, 0.90, 1.78 and 1.18 µg/kg, respectively. The average recoveries of the acetanilide herbicides at spiked concentrations of 10, 50, and 100 µg/kg ranged from 86.9% to 119.0%, and relative standard deviations were equal to or lower than 2.80%.
建立了一种基于金属有机骨架的提取方法，并将其应用于黑芸豆、红芸豆和芸豆中酰胺类除草剂，包括甲草胺、丙草胺、异丙甲草胺和丁草胺的检测。采用乙酸乙酯和正己烷的混合物提取酰胺类除草剂，用金属有机骨架 MIL-101（Zn）对提取液进行吸附和净化。采用高效液相色谱法对四种酰胺类除草剂进行分离和测定。通过单因素法和正交实验对实验参数进行了评价。该方法可以得到有效的提取和净化。甲草胺、丙草胺、异丙甲草胺和丁草胺的检测限分别为 0.58、0.90、1.78 和 1.18μg/kg。在 10、50 和 100μg/kg 浓度下，酰胺类除草剂的平均回收率为 86.9%至 119.0%，相对标准偏差等于或低于 2.80%。
