InGeneron, Inc., Houston, TX, United States of America.
Heart and Vascular Institute, Department of Medicine, Tulane University Health Science Center, New Orleans, LA, United States of America.
PLoS One. 2019 Sep 3;14(9):e0221457. doi: 10.1371/journal.pone.0221457. eCollection 2019.
Freshly isolated, uncultured, autologous adipose derived regenerative cells (ADRCs) have emerged as a promising tool for regenerative cell therapy. The Transpose RT system (InGeneron, Inc., Houston, TX, USA) is a system for isolating ADRCs from adipose tissue, commercially available in Europe as a CE-marked medical device and under clinical evaluation in the United States. This system makes use of the proprietary, enzymatic Matrase Reagent for isolating cells. The present study addressed the question whether the use of Matrase Reagent influences cell yield, cell viability, live cell yield, biological characteristics, physiological functions or structural properties of the ADRCs in final cell suspension. Identical samples of subcutaneous adipose tissue from 12 subjects undergoing elective lipoplasty were processed either with or without the use of Matrase Reagent. Then, characteristics of the ADRCs in the respective final cell suspensions were evaluated. Compared to non-enzymatic isolation, enzymatic isolation resulted in approximately twelve times higher mean cell yield (i.e., numbers of viable cells/ml lipoaspirate) and approximately 16 times more colony forming units. Despite these differences, cells isolated from lipoaspirate both with and without the use of Matrase Reagent were independently able to differentiate into cells of all three germ layers. This indicates that biological characteristics, physiological functions or structural properties relevant for the intended use were not altered or induced using Matrase Reagent. A comprehensive literature review demonstrated that isolation of ADRCs from lipoaspirate using the Transpose RT system and the Matrase Reagent results in the highest viable cell yield among published data regarding isolation of ADRCs from lipoaspirate.
新鲜分离的、未培养的、自体脂肪来源的再生细胞（ADRCs）已成为再生细胞治疗的有前途的工具。 Transpose RT 系统（InGeneron，Inc.，休斯顿，TX，美国）是一种从脂肪组织中分离 ADRCs 的系统，在欧洲作为 CE 标记的医疗器械商业上可获得，并正在美国进行临床评估。该系统利用专有的酶制剂 Matrase Reagent 来分离细胞。本研究旨在探讨 Matrase Reagent 的使用是否会影响 ADRCs 的细胞产量、细胞活力、活细胞产量、生物学特性、生理功能或最终细胞悬浮液中的结构特性。对 12 名接受选择性吸脂术的受试者的皮下脂肪组织进行相同的处理，分别使用和不使用 Matrase Reagent。然后，评估了各自最终细胞悬浮液中 ADRCs 的特性。与非酶分离相比，酶分离导致平均细胞产量（即每毫升脂肪抽吸物的活细胞数）增加约 12 倍，集落形成单位增加约 16 倍。尽管存在这些差异，但使用和不使用 Matrase Reagent 分离的脂肪抽吸物中的细胞均能够独立分化为三个胚层的细胞。这表明使用 Matrase Reagent 并未改变或诱导与预期用途相关的生物学特性、生理功能或结构特性。全面的文献综述表明，与从脂肪抽吸物中分离 ADRCs 的其他方法相比，使用 Transpose RT 系统和 Matrase Reagent 从脂肪抽吸物中分离 ADRCs 可获得最高的活细胞产量。
