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未修饰的新鲜自体脂肪源性再生细胞与富含生长因子的血浆联合应用改善引导性骨再生：首例人体病例报告及文献综述

Improved guided bone regeneration by combined application of unmodified, fresh autologous adipose derived regenerative cells and plasma rich in growth factors: A first-in-human case report and literature review.

作者信息

Solakoglu Önder, Götz Werner, Kiessling Maren C, Alt Christopher, Schmitz Christoph, Alt Eckhard U

机构信息

External Visiting Lecturer, Dental Department of the University Medical Center Hamburg-Eppendorf, Hamburg 20246, Germany.

Department of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn 53111, Germany.

出版信息

World J Stem Cells. 2019 Feb 26;11(2):124-146. doi: 10.4252/wjsc.v11.i2.124.

DOI:10.4252/wjsc.v11.i2.124
PMID:30842809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6397807/
Abstract

BACKGROUND

Novel strategies are needed for improving guided bone regeneration (GBR) in oral surgery prior to implant placement, particularly in maxillary sinus augmentation (GBR-MSA) and in lateral alveolar ridge augmentation (LRA). This study tested the hypothesis that the combination of freshly isolated, unmodified autologous adipose-derived regenerative cells (UA-ADRCs), fraction 2 of plasma rich in growth factors (PRGF-2) and an osteoinductive scaffold (OIS) (UA-ADRC/PRGF-2/OIS) is superior to the combination of PRGF-2 and the same OIS alone (PRGF-2/OIS) in GBR-MSA/LRA.

CASE SUMMARY

A 79-year-old patient was treated with a bilateral external sinus lift procedure as well as a bilateral lateral alveolar ridge augmentation. GBR-MSA/LRA was performed with UA-ADRC/PRGF-2/OIS on the right side, and with PRGF-2/OIS on the left side. Biopsies were collected at 6 wk and 34 wk after GBR-MSA/LRA. At the latter time point implants were placed. Radiographs (32 mo follow-up time) demonstrated excellent bone healing. No radiological or histological signs of inflammation were observed. Detailed histologic, histomorphometric, and immunohistochemical analysis of the biopsies evidenced that UA-ADRC/PRGF-2/OIS resulted in better and faster bone regeneration than PRGF-2/OIS.

CONCLUSION

GBR-MSA with UA-ADRCs, PRGF-2, and an OIS shows effectiveness without adverse effects.

摘要

背景

在种植体植入前的口腔外科手术中，尤其是在上颌窦提升术（GBR-MSA）和外侧牙槽嵴增高术（LRA）中，需要新的策略来改善引导骨再生（GBR）。本研究检验了以下假设：在GBR-MSA/LRA中，新鲜分离、未修饰的自体脂肪来源再生细胞（UA-ADRCs）、富含生长因子的血浆2组分（PRGF-2）和骨诱导支架（OIS）的组合（UA-ADRC/PRGF-2/OIS）优于单独的PRGF-2和相同的OIS组合（PRGF-2/OIS）。

病例摘要

一名79岁患者接受了双侧外提升术以及双侧外侧牙槽嵴增高术。右侧采用UA-ADRC/PRGF-2/OIS进行GBR-MSA/LRA，左侧采用PRGF-2/OIS。在GBR-MSA/LRA后6周和34周采集活检样本。在后者这个时间点植入种植体。X线片（随访32个月）显示骨愈合良好。未观察到炎症的放射学或组织学迹象。对活检样本进行的详细组织学、组织形态计量学和免疫组织化学分析证明，UA-ADRC/PRGF-2/OIS比PRGF-2/OIS能带来更好、更快的骨再生。

结论

GBR-MSA联合UA-ADRCs、PRGF-2和OIS显示出有效性且无不良反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/0b944cc433f2/WJSC-11-124-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/f397b94871be/WJSC-11-124-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/b467e80f519c/WJSC-11-124-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/7caca1caa202/WJSC-11-124-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/35c0e448b7ce/WJSC-11-124-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/dbafe1c4aad7/WJSC-11-124-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/d5180124349c/WJSC-11-124-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/39aa398051ec/WJSC-11-124-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/0b944cc433f2/WJSC-11-124-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/f397b94871be/WJSC-11-124-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/b467e80f519c/WJSC-11-124-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/93bf2ecd72f8/WJSC-11-124-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/7caca1caa202/WJSC-11-124-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/35c0e448b7ce/WJSC-11-124-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/dbafe1c4aad7/WJSC-11-124-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/d5180124349c/WJSC-11-124-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/39aa398051ec/WJSC-11-124-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b6d/6397807/0b944cc433f2/WJSC-11-124-g009.jpg

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