Savinova T A, Bocharova Yu A, Lazareva A V, Chebotar I V, Mayanskiy N A
National Medical Research Center for Children's Health Federal state autonomous institution of the Russian Federation Ministry of Health, Russia, 119296, Moscow, Russia.
Central Research Institute of Epidemiology of the Federal Service on Customers' Rights Protection and Human Well-being Surveillance, 111123, Moscow, Russia.
Klin Lab Diagn. 2019;64(8):497-502. doi: 10.18821/0869-2084-2019-64-8-497-502.
The growing prevalence of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa in nosocomial pathogen populations has been attributed to their clonal spread, and/or horizontal transfer of MBL determinants in mobile genetic elements, including integrons. To characterize the genetic background of the beta-lactamase VIM-2 encoding gene in the population of carbapenemresistant (Carba-R) P. aeruginosa clinical isolates.The detection of class 1 integrons was performed by PCR. Typing of the class 1 integrons containing the bla gene cassette was performed by the PCR-restriction fragment length polymorphism (RFLP) approach followed by sequencing of variable regions of class 1 integrons. Five types of the bla-carrying integrons were identified: ST654-isolates accounting for more than 50% of the Carba-R population harbored In56; ST235-isolates contained In559 (26% Carba-R isolates); ST111-isolates (19% Carba-R isolates) were characterized by carrying In59-like integron; two ST235-isolates harbored In59 and In249 each. Except In56, carrying the only bla-gene cassette, all other identified integron types harbored the genes of resistance to trimethoprim and/or aminoglycosides. No new types of integrons were identified in the P. aeruginosa clinical isolates. The observed correlation of the integron type with specific STs indicates a clonal dissemination of significant resistance determinant producers - ST111, ST654 and ST235 epidemic lines. The features of the integron variable regions can be used for the epidemiological characterization of clinical P. aeruginosa isolates.
在医院病原体群体中，产金属β-内酰胺酶（MBL）的铜绿假单胞菌患病率不断上升，这归因于它们的克隆传播，和/或MBL决定簇在包括整合子在内的可移动遗传元件中的水平转移。为了表征耐碳青霉烯类（Carba-R）铜绿假单胞菌临床分离株群体中β-内酰胺酶VIM-2编码基因的遗传背景。通过聚合酶链反应（PCR）检测1类整合子。通过PCR-限制性片段长度多态性（RFLP）方法对含有bla基因盒的1类整合子进行分型，随后对1类整合子的可变区进行测序。鉴定出了五种携带bla的整合子类型：占Carba-R群体50%以上的ST654分离株携带In56；ST235分离株含有In559（占Carba-R分离株的26%）；ST111分离株（占Carba-R分离株的19%）的特征是携带In59样整合子；两个ST235分离株分别携带In59和In249。除了携带唯一bla基因盒的In56外，所有其他鉴定出的整合子类型都携带对甲氧苄啶和/或氨基糖苷类耐药的基因。在铜绿假单胞菌临床分离株中未鉴定出新型整合子。观察到的整合子类型与特定序列类型（STs）的相关性表明，重要耐药决定簇产生者——ST111、ST654和ST235流行株系存在克隆传播。整合子可变区的特征可用于临床铜绿假单胞菌分离株的流行病学特征分析。
