Knudson W, Toole B P
Department of Biochemistry, Rush/Presbyterian/St Luke's Medical Center, Chicago, Illinois 60612.
J Cell Biochem. 1988 Nov;38(3):165-77. doi: 10.1002/jcb.240380304.
LX-1 human lung carcinoma cells interact with human fibroblasts in culture to cause an increase in hyaluronate production (Knudson et al: Proceedings of the National Academy of Sciences of the United States of America 81:6767, 1984). It is shown here that a similar increase in hyaluronate production also occurs when membranes derived from LX-1 cells, or detergent extracts thereof, are added to cultures of the human fibroblasts. However, no stimulation occurs when membranes or extracts from fibroblasts are added to cultures of the LX-1 cells. The hyaluronate stimulatory factor present in the detergent extracts is a heat- and trypsin-sensitive protein, requires more than 12 h for its action on fibroblasts, causes an elevation in hyaluronate synthetase activity in membranes derived from the fibroblasts, and can be reconstituted into artificial lipid vesicles. Thus, it is concluded that the stimulatory factor is a membrane-bound protein present on the surface of the LX-1 cells and that it interacts with fibroblasts to induce increased hyaluronate synthesis.
LX-1人肺癌细胞在培养过程中与人成纤维细胞相互作用，导致透明质酸盐产量增加（Knudson等人：《美国国家科学院院刊》81:6767，1984）。本文表明，当将来自LX-1细胞的膜或其去污剂提取物添加到人成纤维细胞培养物中时，透明质酸盐产量也会出现类似的增加。然而，当将成纤维细胞的膜或提取物添加到LX-1细胞培养物中时，不会产生刺激作用。去污剂提取物中存在的透明质酸盐刺激因子是一种对热和胰蛋白酶敏感的蛋白质，对成纤维细胞的作用需要超过12小时，会导致成纤维细胞衍生膜中透明质酸合成酶活性升高，并且可以重构到人工脂质小泡中。因此，得出结论，刺激因子是存在于LX-1细胞表面的一种膜结合蛋白，它与成纤维细胞相互作用以诱导透明质酸合成增加。
