Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Espinardo, Murcia, Spain.
Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Espinardo, Murcia, Spain.
Domest Anim Endocrinol. 2020 Jan;70:106380. doi: 10.1016/j.domaniend.2019.07.008. Epub 2019 Jul 27.
Adiponectin (ADP) is an adipokine secreted by adipose tissue with anti-inflammatory, antiatherogenic, and antidiabetic properties. In human serum, it is presented as three different forms: low molecular weight (LMW), medium molecular weight (MMW), and high molecular weight (HMW). High molecular weight isomer is the most active form of ADP and is more closely related with obesity-induced insulin resistance and metabolic syndrome than total ADP. Selective protease treatment can be used in humans to isolate the different ADP isoforms but this has not been applied in any veterinary species. Therefore, the objective of this study was to evaluate if the selective protease digestion is able to differentiate serum ADP isomers in dog samples, and if these isomers could change in obese dogs after a weight loss program. A Western blotting analysis confirmed that digestion with protease K showed only the HMW forms of ADP, whereas the use of protease A showed the HMW and MMW forms. This specific protease digestion was applied to serum obtained from 14 obese beagle dogs before and after a weight loss program and total ADP, HMW, and LMW forms increased significantly after the weight reduction. In conclusion, the use of selective protease digestion can be applied in canine serum as a procedure for detecting the different ADP isomers. In addition, by this procedure, it was showed that the HMW and LMW forms were increased after a weight loss program in our experimental conditions.
脂联素（ADP）是一种由脂肪组织分泌的脂肪因子，具有抗炎、抗动脉粥样硬化和抗糖尿病的特性。在人血清中，它以三种不同的形式存在：低分子量（LMW）、中分子量（MMW）和高分子量（HMW）。高分子量异构体是 ADP 最活跃的形式，与肥胖引起的胰岛素抵抗和代谢综合征的关系比总 ADP 更密切。选择性蛋白酶处理可用于人类分离不同的 ADP 异构体，但尚未在任何兽医物种中应用。因此，本研究的目的是评估选择性蛋白酶消化是否能够区分犬血清 ADP 异构体，以及这些异构体在肥胖犬减肥计划后是否会发生变化。Western 印迹分析证实，用蛋白酶 K 消化仅显示 ADP 的高分子量形式，而使用蛋白酶 A 则显示高分子量和中分子量形式。这种特定的蛋白酶消化应用于 14 只肥胖比格犬减肥前后的血清中，总 ADP、高分子量和低分子量形式在减肥后显著增加。总之，选择性蛋白酶消化可应用于犬血清中，作为检测不同 ADP 异构体的方法。此外，通过该程序，在我们的实验条件下，显示出在减肥计划后 HMW 和 LMW 形式增加。
