水相双液相系统结合超声细胞破碎提取和 HPLC 在同时分离和分析龙葵未成熟果实中的龙葵碱和龙葵多糖中的应用。
The application of an aqueous two-phase system combined with ultrasonic cell disruption extraction and HPLC in the simultaneous separation and analysis of solanine and Solanum nigrum polysaccharide from Solanum nigrum unripe fruit.
机构信息
Key Laboratory of Preparation and Application of Environmental Friendly Materials, Jilin Normal University, Ministry of Education, Siping 136000, China; Jilin Provincial Key Laboratory for Numerical Simulation, Jilin Normal University, 1301 Haifeng Street, Siping 136000, China.
Key Laboratory of Preparation and Application of Environmental Friendly Materials, Jilin Normal University, Ministry of Education, Siping 136000, China; Jilin Provincial Key Laboratory for Numerical Simulation, Jilin Normal University, 1301 Haifeng Street, Siping 136000, China.
出版信息
Food Chem. 2020 Jan 30;304:125383. doi: 10.1016/j.foodchem.2019.125383. Epub 2019 Aug 28.
An aqueous two-phase system was used in conjunction with ultrasonic cell disruption to extract and separate solanine (mainly solasonine and solamargine) and Solanum nigrum polysaccharide from Solanum nigrum unripe fruit. The optimized conditions of the present study were determined by a single-factor experiment and a multifactor experiment. The concentration of ethanol was set at 60% and the duration of the ultrasonic cell disruption extraction was 50 min. In the ethanol-KCO aqueous two-phase separation system, the concentration of ethanol was 36%, the concentration of KCO was 0.21 mg·mL, and the temperature was 15 °C. The solasonine and solamargine were determined by high-performance liquid chromatography, and the Solanum nigrum polysaccharide was determined by an ultraviolet-visible spectrophotometer in accordance with the phenol-sulfuric acid method. xUnder optimized conditions, the average extraction efficiencies of solasonine, solamargine and Solanum nigrum polysaccharide were 95.86%, 95.95% and 96.95%, respectively, and the average separation efficiencies of solasonine, solamargine and Solanum nigrum polysaccharide were 2.07 mg·g, 2.05 mg·g and 8.15 mg·g, respectively.
采用双水相体系结合超声细胞破碎法从未成熟的龙葵果实中提取分离龙葵碱（主要为澳洲茄碱和澳洲茄边碱）和龙葵多糖。通过单因素实验和多因素实验确定了本研究的最佳条件。乙醇浓度设定为 60%，超声细胞破碎提取时间为 50min。在乙醇-KCO 双水相分离体系中，乙醇浓度为 36%，KCO 浓度为 0.21mg·mL，温度为 15°C。采用高效液相色谱法测定澳洲茄碱和澳洲茄边碱的含量，采用紫外可见分光光度法依照苯酚-硫酸法测定龙葵多糖的含量。在优化条件下，龙葵碱、澳洲茄边碱和龙葵多糖的平均提取率分别为 95.86%、95.95%和 96.95%，龙葵碱、澳洲茄边碱和龙葵多糖的平均分离率分别为 2.07mg·g、2.05mg·g 和 8.15mg·g。
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