Biscop Eline, Lin Abraham, Boxem Wilma Van, Loenhout Jinthe Van, Backer Joey De, Deben Christophe, Dewilde Sylvia, Smits Evelien, Bogaerts And Annemie
PLASMANT Research Group, Department of Chemistry, University of Antwerp, Antwerp 2610, Belgium.
Center for Oncological Research, University of Antwerp, Antwerp 2610, Belgium.
Cancers (Basel). 2019 Sep 1;11(9):1287. doi: 10.3390/cancers11091287.
Increasing the selectivity of cancer treatments is attractive, as it has the potential to reduce side-effects of therapy. Cold atmospheric plasma (CAP) is a novel cancer treatment that disrupts the intracellular oxidative balance. Several reports claim CAP treatment to be selective, but retrospective analysis of these studies revealed discrepancies in several biological factors and culturing methods. Before CAP can be conclusively stated as a selective cancer treatment, the importance of these factors must be investigated. In this study, we evaluated the influence of the cell type, cancer type, and cell culture medium on direct and indirect CAP treatment. Comparison of cancerous cells with their non-cancerous counterparts was performed under standardized conditions to determine selectivity of treatment. Analysis of seven human cell lines (cancerous: A549, U87, A375, and Malme-3M; non-cancerous: BEAS-2B, HA, and HEMa) and five different cell culture media (DMEM, RPMI1640, AM, BEGM, and DCBM) revealed that the tested parameters strongly influence indirect CAP treatment, while direct treatment was less affected. Taken together, the results of our study demonstrate that cell type, cancer type, and culturing medium must be taken into account before selectivity of CAP treatment can be claimed and overlooking these parameters can easily result in inaccurate conclusions of selectivity.
提高癌症治疗的选择性很有吸引力，因为它有可能减少治疗的副作用。冷大气等离子体（CAP）是一种新型的癌症治疗方法，它会破坏细胞内的氧化平衡。有几份报告称CAP治疗具有选择性，但对这些研究的回顾性分析显示，在几个生物学因素和培养方法上存在差异。在能够确凿地认定CAP为一种选择性癌症治疗方法之前，必须对这些因素的重要性进行研究。在本研究中，我们评估了细胞类型、癌症类型和细胞培养基对直接和间接CAP治疗的影响。在标准化条件下，将癌细胞与其对应的非癌细胞进行比较，以确定治疗的选择性。对七种人类细胞系（癌细胞系：A549、U87、A375和Malme-3M；非癌细胞系：BEAS-2B、HA和HEMa）和五种不同的细胞培养基（DMEM、RPMI1640、AM、BEGM和DCBM）的分析表明，所测试的参数对间接CAP治疗有很大影响，而直接治疗受到的影响较小。综上所述，我们的研究结果表明，在声称CAP治疗具有选择性之前，必须考虑细胞类型、癌症类型和培养基，而忽视这些参数很容易导致关于选择性的不准确结论。
