Faculty of Sport and Health Science, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga 525-8577, Japan.
Research Fellow of Japan Society for the Promotion of Science, 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan.
Nutrients. 2019 Sep 2;11(9):2049. doi: 10.3390/nu11092049.
Our previous study showed that lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α production is inhibited by acute exhaustive exercise in mice, leading to transient immunodepression after exercise. (SC), an edible mushroom, has immunopotentiative properties. This study aimed to clarify the effects of SC intake on reduced LPS-induced TNF-α production upon exhaustive exercise in mice. Male C3H/HeN mice were randomly divided into three groups: normal chow intake + resting sedentary, normal chow intake + acute exhaustive treadmill running exercise, and SC intake (chow containing 5% SC powder for 8 weeks) + the exhaustive exercise groups. Each group was injected with LPS immediately after the exhaustive exercise or rest. Plasma and tissue TNF-α levels were significantly decreased by exhaustive exercise. However, this reduction of the TNF-α level was partially attenuated in the plasma and small intestine by SC intake. Although levels of TLR4 and MyD88 protein expression were significantly decreased in tissues by exhaustive exercise, the reduction of TLR4 and MyD88 levels in the small intestine was partially attenuated by SC intake. These results suggest that SC intake attenuates exhaustive exercise-induced reduction of TNF-α production via the retention of TLR4 and MyD88 expression in the small intestine.
我们之前的研究表明，脂多糖（LPS）诱导的肿瘤坏死因子（TNF）-α产生在小鼠中被急性耗竭运动抑制，导致运动后短暂的免疫抑制。香菇（SC）具有免疫增强特性。本研究旨在阐明 SC 摄入对减少 LPS 诱导的 TNF-α产生的影响在小鼠剧烈运动后。雄性 C3H/HeN 小鼠随机分为三组：正常饲料摄入+休息久坐、正常饲料摄入+急性耗竭跑步机运动和 SC 摄入（含 5% SC 粉的饲料 8 周）+剧烈运动组。每组在剧烈运动或休息后立即注射 LPS。血浆和组织 TNF-α水平因剧烈运动而显著降低。然而，SC 摄入部分减轻了血浆和小肠中 TNF-α水平的降低。尽管组织中 TLR4 和 MyD88 蛋白表达水平因剧烈运动而显著降低，但 SC 摄入部分减轻了小肠中 TLR4 和 MyD88 水平的降低。这些结果表明，SC 摄入通过保留 TLR4 和 MyD88 在小肠中的表达，减轻了剧烈运动引起的 TNF-α产生减少。
