Imagawa M, Ohtsuka M, Nakatani Y, Oku Y, el-Belbasi H I, Nishihara T, Kondo M
Faculty of Pharmaceutical Sciences, Osaka University Suita.
Microbiol Immunol. 1988;32(10):991-8. doi: 10.1111/j.1348-0421.1988.tb01463.x.
An immunochemical staining technique for the spore coat proteins of Bacillus megaterium ATCC 12872 was developed using colloidal gold as a second antibody. For reducing the non-specific immunogold binding and increasing the specific binding, the affinity-purified IgG was used as a first antibody. In sporulating cells at t10, gold particles were found not only in the spore coat but also in the mother cell cytoplasm, suggesting that some coat proteins were synthesized in the cytoplasm. Use of the specific affinity-purified antibody to 48K-protein demonstrated that this protein was one of the components of the outer coat.
