Nishihara T, Tomita M, Yamanaka N, Ichikawa T, Kondo M
Microbiol Immunol. 1980;24(2):105-12. doi: 10.1111/j.1348-0421.1980.tb00568.x.
Properties of alkali-soluble components from the spore coat of Bacillus megaterium were examined by physicochemical methods. They were composed of acidic polypeptides of various molecular weights with small amounts of phosphorus and sugar. They were allowed to dissociate to unit components by incubation with SDS. The major component was partially purified by gel filtration, and shown to have a mean molecular weight of about 11,000.
用物理化学方法检测了巨大芽孢杆菌芽孢衣中碱溶性成分的性质。它们由各种分子量的酸性多肽组成,并含有少量的磷和糖。通过与十二烷基硫酸钠(SDS)温育使其解离为单一成分。主要成分通过凝胶过滤进行了部分纯化,其平均分子量约为11,000。