Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Plant Sci. 2019 Oct;287:110177. doi: 10.1016/j.plantsci.2019.110177. Epub 2019 Jun 25.
The control of epidermal cell fate is an elaborate molecular process mediated by the TTG1-bHLH-MYB regulatory complex. In this study, we isolated PaMYB82 from London plane. PaMYB82 was revealed to be a nuclear-localized transcription activator and was found to be expressed ubiquitously in the tissues of roots, stems, leaves, cotyledons and hypocotyls. Expression of the PaMYB82 gene under the control of the viral CaMV35S promoter caused a nearly glabrous phenotype in wild type Arabidopsis and can partially rescue the gl1 mutant phenotype. Protein interaction analysis revealed that PaMYB82 physically interacts with PaGL3 and itself, in addition, PaMYB82 could interact with trichome related bHLH transcription factors AtGL3, AtEGL3 and AtMYC1. Expression levels of AtGL2, AtTTG2 and several R3 MYB genes were greatly increased in 35S::PaMYB82 lines. The expression of AtMYB23 was reduced in 35S::PaMYB82 transgenic lines, whereas, expression levels of AtGL1 remained unchanged indicating that differences in the transcriptional regulation of AtMYB23 and AtGL1 during trichome development. Together, the data presented here indicate that PaMYB82 encodes a functional R2R3 MYB transcription factor which can control the initiation of Arabidopsis trichome development.
表皮细胞命运的控制是一个由 TTG1-bHLH-MYB 调控复合物介导的精细分子过程。在本研究中，我们从伦敦悬铃木中分离出 PaMYB82。PaMYB82 被揭示为一种核定位转录激活因子，在根、茎、叶、子叶和下胚轴的组织中广泛表达。在病毒 CaMV35S 启动子的控制下，PaMYB82 基因的表达导致野生型拟南芥几乎无毛的表型，并能部分挽救 gl1 突变体的表型。蛋白相互作用分析表明，PaMYB82 与 PaGL3 和自身发生物理相互作用，此外，PaMYB82 还可以与毛状体相关的 bHLH 转录因子 AtGL3、AtEGL3 和 AtMYC1 相互作用。在 35S::PaMYB82 系中，AtGL2、AtTTG2 和几个 R3 MYB 基因的表达水平大大增加。在 35S::PaMYB82 转基因系中，AtMYB23 的表达减少，而 AtGL1 的表达水平保持不变，表明在毛状体发育过程中 AtMYB23 和 AtGL1 的转录调控存在差异。总之，本文提供的数据表明，PaMYB82 编码一种功能性的 R2R3 MYB 转录因子，可控制拟南芥毛状体发育的启动。
