Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland.
Antimicrob Agents Chemother. 2019 Oct 22;63(11). doi: 10.1128/AAC.01403-19. Print 2019 Nov.
A plasmid-located fosfomycin resistance gene, , was identified from a CTX-M-15-producing isolate recovered from urine. Identification of this gene was obtained by whole-genome sequencing. It encoded FosA8, which shares 79% and 78% amino acid identity with the most closely related FosA2 and FosA1 enzymes, respectively. The gene was located on a transferable 50-kb plasmid of IncN type encoding high-level resistance to fosfomycin. analysis and cloning experiments identified analogues (99% identity) in the genome of , which is an enterobacterial species with natural resistance to fosfomycin. This finding adds to the list of enterobacterial species that are a reservoir of -like genes which have been captured from the chromosome of a progenitor and are then acquired by .
从尿液中分离出一株产 CTX-M-15 的 ，我们在其质粒上发现了一种编码磷霉素抗性的 fosfomycin 耐药基因 。我们通过全基因组测序确定了该基因的存在。它编码的 FosA8 与最接近的 FosA2 和 FosA1 酶分别具有 79%和 78%的氨基酸同一性。该基因位于可转移的 50kb IncN 型质粒上，该质粒对磷霉素具有高水平的耐药性。 分析和克隆实验在 的基因组中鉴定出了类似物（99%的同一性）， 是一种天然对磷霉素具有抗性的肠杆菌属细菌。这一发现增加了肠杆菌属物种的名单，这些物种是 -样基因的库，这些基因已经从一个前体的染色体上捕获，并被 获得。
