白细胞介素-17 受体 C 基因多态性降低缺血性脑卒中的治疗效果并促进不良预后。
Interleukin-17 receptor C gene polymorphism reduces treatment effect and promotes poor prognosis of ischemic stroke.
机构信息
Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.
Department of Neurology, First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang 471003, China.
出版信息
Biosci Rep. 2019 Oct 30;39(10). doi: 10.1042/BSR20190435.
OBJECTIVE
To study the relationship between Interleukin-17 receptor C (IL-17RC) gene polymorphism and ischemic stroke (IS).
METHODS
Three hundred cases of IS patients and 300 cases of the healthy controls were selected. Serum of IS patients and the controls was collected. The relative mRNA levels of IL-17, IL-17RC, IL-6, IL-8, G-CSF and granulocyte-macrophage colony stimulating factor (GM-CSF) by qRT-PCR. The protein expression of IL-17 and IL-17RC was determined by Western blotting. IL-17RC genotype was identified by PCR amplification. The proportion of IL-17RC, SNP and re37511 in IS and control group was determined. The treatment effect on IS and prognosis of patients with IL-17RC, SNP and re37511 was compared.
RESULTS
The relative mRNA levels of IL-17, IL-17RC, IL-6, IL-8, G-CSF and GM-CSF in IS group were significantly higher than the control group. The protein expression of IL-17 and IL-17RC in IS group was also markedly higher than the control group. The proportion of IL-17RC re37511 in IS group was much larger than control group and proportion of IL-17RC much less. The percent of poor treatment effect in re37511 was much larger than IL-17RC. The percent of death and recrudescence in patients with IL-17RC re37511 was the highest.
CONCLUSION
IS up-regulates the expression of IL-17 and IL-17RC. IL-17RC re37511 indicates the patients have a poorer treatment effect and prognosis.
目的
研究白细胞介素-17 受体 C（IL-17RC）基因多态性与缺血性脑卒中（IS）的关系。
方法
选择 300 例 IS 患者和 300 例健康对照者。采集 IS 患者和对照组的血清。采用 qRT-PCR 检测 IL-17、IL-17RC、IL-6、IL-8、G-CSF 和粒细胞-巨噬细胞集落刺激因子（GM-CSF）的相对 mRNA 水平。采用 Western blot 法测定 IL-17 和 IL-17RC 的蛋白表达。采用 PCR 扩增法鉴定 IL-17RC 基因型。确定 IS 组和对照组中 IL-17RC、SNP 和 re37511 的比例。比较 IL-17RC、SNP 和 re37511 对 IS 患者的治疗效果和预后。
结果
IS 组的 IL-17、IL-17RC、IL-6、IL-8、G-CSF 和 GM-CSF 的相对 mRNA 水平明显高于对照组。IS 组 IL-17 和 IL-17RC 的蛋白表达也明显高于对照组。IS 组的 IL-17RC re37511 比例明显大于对照组，而 IL-17RC 比例明显小于对照组。re37511 的治疗效果不良率明显大于 IL-17RC。IL-17RC re37511 患者的死亡率和复发率最高。
结论
IS 上调 IL-17 和 IL-17RC 的表达。IL-17RC re37511 表明患者的治疗效果和预后较差。
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