Department of Pharmacology & Toxicology, University of Toronto, Toronto, Ontario, Canada.
Research Imaging Centre, Centre for Addiction and Mental Health, Toronto, Ontario, Canada.
Sci Rep. 2019 Sep 3;9(1):12695. doi: 10.1038/s41598-019-49180-3.
Altered mitochondrial electron transport chain function has been implicated in the pathophysiology and etiology of schizophrenia. To date, our previously published study (i.e. first cohort) is still the only study to demonstrate that mitochondrial electron transport chain is not altered in white blood cells from individuals at clinical high risk for psychosis. Here, we aimed to replicate our previous findings with an independent set of samples and validate the levels of mitochondrial complex I-V content in individuals at clinical high risk for psychosis. We demonstrated that the second cohort (i.e. validation cohort) expressed similar results as the first cohort. We combined the first cohort study with the second cohort and once more validated a lack of differential levels in mitochondrial complex I-V content between the two groups. In addition, we were able to validate a correlation between complex III content and prodromal negative symptom severity when the two cohorts studies were combined. Additionally, a correlation between complex V content and prodromal disorganization symptom severity was found when the two cohorts were combined. In conclusion, our results showed that dysfunction of the mitochondrial electron transport chain is not detected in peripheral blood mononuclear cells of individuals in the putative prodromal stage of schizophrenia.
线粒体电子传递链功能的改变与精神分裂症的病理生理学和病因学有关。迄今为止，我们之前发表的研究（即第一队列）仍然是唯一一项表明精神病高危个体的白细胞中线粒体电子传递链没有改变的研究。在这里，我们旨在用一组独立的样本复制我们之前的发现，并验证精神病高危个体中线粒体复合物 I-V 含量的水平。我们证明了第二队列（即验证队列）的结果与第一队列相似。我们将第一队列研究与第二队列相结合，再次验证了两组之间线粒体复合物 I-V 含量没有差异。此外，当两个队列的研究结合在一起时，我们能够验证复合物 III 含量与前驱阴性症状严重程度之间的相关性。此外，当两个队列结合在一起时，发现复合物 V 含量与前驱性紊乱症状严重程度之间存在相关性。总之，我们的结果表明，在精神分裂症前驱期个体的外周血单核细胞中未检测到线粒体电子传递链功能障碍。
