Hirata Kaori, Takagi Kyoko, Yamada Tetsuya, Sayama Takashi, Anai Toyoaki, Kikuchi Akio, Ishimoto Masao
Tohoku Agricultural Research Center, National Agriculture and Food Research Organization (NARO), 297 Uenodai, Kariwano, Daisen, Akita 019-2112, Japan.
Tohoku Agricultural Research Center NARO, 50 Harajukuminami, Arai, Fukushima 960-2156, Japan.
Breed Sci. 2019 Jun;69(2):345-351. doi: 10.1270/jsbbs.18091. Epub 2019 Apr 27.
Food contamination by cadmium (Cd) is a serious threat to human health. Thus, it is imperative to prevent Cd accumulation in staple crops like soybean. The development of low Cd accumulating cultivars is an effective solution. To this end, it is essential to identify the gene(s) controlling seed Cd accumulation. Although () seems to be associated with Cd accumulation in soybean, it has not been established if it is responsible for seed Cd accumulation. In the present study, the effect of on seed Cd accumulation in soybean was validated using three independent mutants isolated from an ethyl methanesulfonate-induced soybean mutant library. Each of mutant had an amino acid substitution in GmHMA3 and segregating progenies were developed by crossing the original cultivar with each of the three mutants. The relationship between these three mutations and seed Cd accumulation was investigated. While two of them significantly increased seed Cd accumulation corresponding to previous reports of a natural missense mutation in , the other slightly decreased seed Cd accumulation. Overall, these results indicate that is responsible for seed Cd accumulation in soybean.
镉（Cd）对食品的污染是对人类健康的严重威胁。因此，必须防止镉在大豆等主要作物中积累。培育低镉积累品种是一种有效的解决办法。为此，识别控制种子镉积累的基因至关重要。尽管（）似乎与大豆中的镉积累有关，但尚未确定它是否负责种子镉积累。在本研究中，利用从甲基磺酸乙酯诱导的大豆突变体文库中分离出的三个独立的（）突变体，验证了（）对大豆种子镉积累的影响。每个突变体在GmHMA3中都有一个氨基酸替换，通过将原始品种与三个突变体分别杂交，培育出分离后代。研究了这三个突变与种子镉积累之间的关系。其中两个突变显著增加了种子镉积累，这与之前关于（）中自然错义突变的报道一致，另一个突变则使种子镉积累略有下降。总体而言，这些结果表明（）负责大豆种子中的镉积累。
