IDM, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain.
Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
Anal Bioanal Chem. 2019 Oct;411(25):6745-6754. doi: 10.1007/s00216-019-02050-3. Epub 2019 Sep 3.
In the literature, there are reports of the utilization of various hydrogels to create generic platforms for protein microarray applications. Here, a novel strategy was developed to obtain high-performance microarrays. In it, a dextran hydrogel is used to covalently immobilize oligonucleotides and proteins. This method employs aqueous solutions of dextran methacrylate (Dx-MA), which is a biocompatible photopolymerizable monomer. Capture probes are immobilized inside the hydrogel via a light-induced thiol-acrylate coupling reaction at the same time as the dextran polymer is formed. Hydrogel microarrays based on this technique were prepared on different surfaces, such as a Blu-ray Disk and polycarbonate or alkene-functionalized glass slides, and these systems showed high probe-loading capabilities and good biorecognition yields. This methodology presents advantages such as a low cost, a short analysis time, a low limit of detection, and multiplexing capabilities, among others. Confocal fluorescence microscopy analysis demonstrated that in these hydrogel-based microarrays, receptor immobilization and the biorecognition event occurred within the hydrogel and not merely on the surface.
在文献中，有利用各种水凝胶来创建蛋白质微阵列应用通用平台的报道。在这里，开发了一种新策略来获得高性能的微阵列。在该策略中，葡聚糖水凝胶用于共价固定寡核苷酸和蛋白质。该方法使用葡聚糖甲基丙烯酰胺（Dx-MA）的水溶液，这是一种生物相容性的光聚合可聚合单体。在形成葡聚糖聚合物的同时，通过光诱导的巯基-丙烯酰基偶联反应将捕获探针固定在水凝胶内部。基于该技术的水凝胶微阵列在不同的表面上进行制备，例如蓝光光盘和聚碳酸酯或烯基官能化玻璃载玻片，这些系统显示出高的探针负载能力和良好的生物识别产率。该方法具有成本低、分析时间短、检测限低和多重检测能力等优点。共聚焦荧光显微镜分析表明，在这些基于水凝胶的微阵列中，受体固定和生物识别事件发生在水凝胶内部，而不仅仅是在表面上。
