血清微小RNA-21基因表达对结直肠癌诊断和预后的评估
Assessment of Serum MicroRNA-21 Gene Expression for Diagnosis and Prognosis of Colorectal Cancer.
作者信息
Ghareib Amal F, Mohamed Randa H, Abd El-Fatah Ahmed R, Saadawy Sara F
机构信息
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Clinical Laboratory Department, Faculty of Applied Medical Science, Taif University, Taif, Saudi Arabia.
出版信息
J Gastrointest Cancer. 2020 Sep;51(3):818-823. doi: 10.1007/s12029-019-00306-w.
BACKGROUND
MicroRNAs (miRNAs) are small, non-coding RNAs that are involved in carcinogenesis through posttranscriptional gene regulatory activity. The current study aimed to evaluate serum miR-21 expression levels as potential biomarkers for diagnosis and prognosis of colorectal cancer (CRC) patients.
METHODS
Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 in serum. At the same time, the sensitivity and specificity of this marker were evaluated by receiver operating characteristic (ROC) curve analysis.
RESULTS
miR-21 expression levels of serum were 3.4 and 1.25 in patient and control, respectively (p < 0.05). The sensitivity and specificity of miR-21 were found to be 95.8% and 91.7%, respectively. The high expression level of serum miR-21 were associated with higher local recurrence, TNM staging, PT staging, venous invasion, liver metastasis, and recurrence (p < 0.05).
CONCLUSION
The results of this study indicated that miR-21 expression levels in serum can be considered as a novel non-invasive biomarker for early detection and prognosis of CRC patients.
背景
微小RNA（miRNA）是一类小的非编码RNA，通过转录后基因调控活性参与致癌过程。本研究旨在评估血清miR-21表达水平作为结直肠癌（CRC）患者诊断和预后的潜在生物标志物。
方法
应用定量实时逆转录聚合酶链反应（RT-PCR）测定血清中miR-21的相对表达水平。同时，通过受试者工作特征（ROC）曲线分析评估该标志物的敏感性和特异性。
结果
患者组和对照组血清miR-21表达水平分别为3.4和1.25（p < 0.05）。miR-21的敏感性和特异性分别为95.8%和91.7%。血清miR-21高表达水平与更高的局部复发、TNM分期、PT分期、静脉侵犯、肝转移和复发相关（p < 0.05）。
结论
本研究结果表明，血清miR-21表达水平可被视为CRC患者早期检测和预后的一种新型非侵入性生物标志物。
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