• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于核酸适配体功能化金纳米十二面体阵列的表面增强拉曼散射检测平台，用于高效同步检测结直肠癌相关微小核糖核酸。

SERS detection platform based on a nucleic acid aptamer-functionalized Au nano-dodecahedron array for efficient simultaneous testing of colorectal cancer-associated microRNAs.

作者信息

Zhang Shuofeng, Chen Fengsong, Zhang Yanqing, Xu Yemin, Wang Lu, Wang Xiya, Jia Long, Chen Yong, Xu Yongcheng, Zhang Zhengrong, Deng Bin

机构信息

Clinical Medical College, Yangzhou University, Yangzhou 225001, China.

Gastroenterology Department, Nantong Haimen People's Hospital, Nantong 226600, China.

出版信息

Biomed Opt Express. 2024 Apr 26;15(5):3366-3381. doi: 10.1364/BOE.520161. eCollection 2024 May 1.

DOI:10.1364/BOE.520161
PMID:38855705
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11161369/
Abstract

A surface-enhanced Raman scattering (SERS) detection platform was constructed based on Au nano-dodecahedrons (AuNDs) functionalized with nucleic acid aptamer-specific binding and self-assembly techniques. SERS labels were prepared by modifying Raman signaling molecules and complementary aptamer chains and were bound on the aptamer-functionalized AuNDs array. Using this protocol, the limits of detection (LODs) of miR-21 and miR-18a in the serum were 6.8 pM and 7.6 pM, respectively, and the detection time was 5 min. Additionally, miR-21 and miR-18a were detected in the serum of a mouse model of colorectal cancer. The results of this protocol were consistent with quantitative real-time polymerase chain reaction (qRT-PCR). This method provides an efficient and rapid method for the simultaneous testing of miRNAs, which has great potential clinical value for the early detection of colorectal cancer (CRC).

摘要

基于用核酸适配体特异性结合和自组装技术功能化的金纳米十二面体（AuNDs）构建了一种表面增强拉曼散射（SERS）检测平台。通过修饰拉曼信号分子和互补适配体链制备SERS标记物，并将其结合在适配体功能化的AuNDs阵列上。使用该方案，血清中miR-21和miR-18a的检测限（LOD）分别为6.8 pM和7.6 pM，检测时间为5分钟。此外，在结直肠癌小鼠模型的血清中检测到了miR-21和miR-18a。该方案的结果与定量实时聚合酶链反应（qRT-PCR）一致。该方法为miRNAs的同时检测提供了一种高效、快速的方法，对结直肠癌（CRC）的早期检测具有巨大的潜在临床价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/9b3a45ee4c51/boe-15-5-3366-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/fd4723a0084f/boe-15-5-3366-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/ab93728d508c/boe-15-5-3366-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/76dccbcf38c3/boe-15-5-3366-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/4bcf0a2a2c02/boe-15-5-3366-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/e1a5b8319139/boe-15-5-3366-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/2b9eefa6e041/boe-15-5-3366-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/8941c3860827/boe-15-5-3366-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/9b3a45ee4c51/boe-15-5-3366-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/fd4723a0084f/boe-15-5-3366-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/ab93728d508c/boe-15-5-3366-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/76dccbcf38c3/boe-15-5-3366-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/4bcf0a2a2c02/boe-15-5-3366-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/e1a5b8319139/boe-15-5-3366-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/2b9eefa6e041/boe-15-5-3366-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/8941c3860827/boe-15-5-3366-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b86/11161369/9b3a45ee4c51/boe-15-5-3366-g008.jpg

相似文献

1
SERS detection platform based on a nucleic acid aptamer-functionalized Au nano-dodecahedron array for efficient simultaneous testing of colorectal cancer-associated microRNAs.基于核酸适配体功能化金纳米十二面体阵列的表面增强拉曼散射检测平台，用于高效同步检测结直肠癌相关微小核糖核酸。
Biomed Opt Express. 2024 Apr 26;15(5):3366-3381. doi: 10.1364/BOE.520161. eCollection 2024 May 1.
2
LoC-SERS platform for rapid and sensitive detection of colorectal cancer protein biomarkers.用于快速灵敏检测结直肠癌蛋白生物标志物的 LOC-SERS 平台。
Talanta. 2024 Apr 1;270:125563. doi: 10.1016/j.talanta.2023.125563. Epub 2023 Dec 19.
3
Catalytic Hairpin Self-Assembly-Based SERS Sensor Array for the Simultaneous Measurement of Multiple Cancer-Associated miRNAs.基于催化发夹自组装的表面增强拉曼散射传感器阵列用于同时检测多种癌症相关微小核糖核酸
ACS Sens. 2020 Dec 24;5(12):4009-4016. doi: 10.1021/acssensors.0c01876. Epub 2020 Dec 7.
4
Aptamer-Based Functionalized SERS Biosensor for Rapid and Ultrasensitive Detection of Gastric Cancer-Related Biomarkers.基于适配体的功能化 SERS 生物传感器用于快速超灵敏检测胃癌相关生物标志物。
Int J Nanomedicine. 2023 Dec 11;18:7523-7532. doi: 10.2147/IJN.S434854. eCollection 2023.
5
3D hierarchic interfacial assembly of Au nanocage@Au along with IS-AgMNPs for simultaneous, ultrasensitive, reliable, and quantitative SERS detection of colorectal cancer related miRNAs.3D 分层界面组装 Au 纳米笼@Au 与 IS-AgMNPs，用于同时、超灵敏、可靠和定量的结直肠癌相关 miRNA 的 SERS 检测。
Biosens Bioelectron. 2024 Mar 15;248:115993. doi: 10.1016/j.bios.2023.115993. Epub 2023 Dec 30.
6
Au nano-cone array for SERS detection of associated miRNA in lymphoma patients.金纳米锥阵列用于淋巴瘤患者相关 miRNA 的 SERS 检测。
Mikrochim Acta. 2023 Dec 19;191(1):40. doi: 10.1007/s00604-023-06095-1.
7
Sensitive and specific detection of clinical bacteria via vancomycin-modified FeO@Au nanoparticles and aptamer-functionalized SERS tags.通过万古霉素修饰的FeO@Au纳米颗粒和适配体功能化的表面增强拉曼光谱标签对临床细菌进行灵敏且特异的检测。
J Mater Chem B. 2018 Jun 14;6(22):3751-3761. doi: 10.1039/c8tb00504d. Epub 2018 May 25.
8
A LoC-SERS platform based on triple signal amplification for highly sensitive detection of colorectal cancer miRNAs.基于三重信号放大的 LOC-SERS 平台用于高灵敏度检测结直肠癌 miRNA。
Anal Methods. 2023 Aug 24;15(33):4194-4203. doi: 10.1039/d3ay01006f.
9
An aptamer and Au/Si CCA based SERS sensor for ultra-sensitive detection of Vimentin during EMT in gastric cancer.一种基于适配体和金/硅壳核结构的表面增强拉曼散射传感器，用于超灵敏检测胃癌上皮-间质转化过程中的波形蛋白。
Front Bioeng Biotechnol. 2023 Dec 7;11:1310258. doi: 10.3389/fbioe.2023.1310258. eCollection 2023.
10
An ultrasensitive and dual-recognition SERS biosensor based on FeO@Au-Teicoplanin and aptamer functionalized Au@Ag nanoparticles for detection of Staphylococcus aureus.基于 FeO@Au-Teicoplanin 和适配体功能化 Au@Ag 纳米粒子的超灵敏双识别 SERS 生物传感器用于检测金黄色葡萄球菌。
Talanta. 2022 Dec 1;250:123648. doi: 10.1016/j.talanta.2022.123648. Epub 2022 Jun 3.

本文引用的文献

1
SERS-based methods for the detection of genomic biomarkers of cancer.基于 SERS 的癌症基因组生物标志物检测方法。
Talanta. 2024 Jan 15;267:125198. doi: 10.1016/j.talanta.2023.125198. Epub 2023 Sep 15.
2
Super-Resolution SERS Spectral Bioimaging.超分辨率表面增强拉曼光谱生物成像
Proc SPIE Int Soc Opt Eng. 2022 Aug;12203. doi: 10.1117/12.2632824. Epub 2022 Oct 3.
3
Detection of miRNAs.miRNAs 的检测。
Methods Mol Biol. 2023;2630:1-11. doi: 10.1007/978-1-0716-2982-6_1.
4
Programmable Microparticle Array for In Situ Modification and Multiple miRNA Detection.用于原位修饰和多重微小RNA检测的可编程微粒阵列
ACS Sens. 2022 Dec 23;7(12):3654-3659. doi: 10.1021/acssensors.2c02158. Epub 2022 Nov 30.
5
Printed Electrochemical Strip for the Detection of miRNA-29a: A Possible Biomarker Related to Alzheimer's Disease.用于检测 miRNA-29a 的电化学条带：与阿尔茨海默病相关的潜在生物标志物。
Anal Chem. 2022 Nov 15;94(45):15558-15563. doi: 10.1021/acs.analchem.2c03542. Epub 2022 Nov 1.
6
Self-Calibration 3D Hybrid SERS Substrate and Its Application in Quantitative Analysis.自校准 3D 混合 SERS 基底及其在定量分析中的应用。
Anal Chem. 2022 Jul 12;94(27):9578-9585. doi: 10.1021/acs.analchem.2c00436. Epub 2022 Jun 30.
7
Aptamers used for molecular imaging and theranostics - recent developments.适体在分子影像和治疗诊断学中的应用——最新进展。
Theranostics. 2022 May 13;12(9):4010-4050. doi: 10.7150/thno.72949. eCollection 2022.
8
A dual-functional fluorescent biosensor based on enzyme-involved catalytic hairpin assembly for the detection of APE1 and miRNA-21.基于酶参与的催化发夹组装的双重功能荧光生物传感器用于检测 APE1 和 miRNA-21。
Analyst. 2022 Jun 13;147(12):2834-2842. doi: 10.1039/d2an00108j.
9
Active site-dominated electromagnetic enhancement of surface-enhanced Raman spectroscopy (SERS) on a Cu triangle plate.铜三角板上基于活性位点主导的表面增强拉曼光谱（SERS）的电磁增强
RSC Adv. 2020 Nov 18;10(69):42030-42037. doi: 10.1039/d0ra08477h. eCollection 2020 Nov 17.
10
Detection of a miRNA biomarker for cancer diagnosis using SERS tags and magnetic separation.利用表面增强拉曼散射（SERS）标签和磁分离技术检测用于癌症诊断的miRNA生物标志物。
Anal Methods. 2022 May 27;14(20):1938-1945. doi: 10.1039/d2ay00210h.