Brunner G, Tegtmeier F, Kirk D N, Wynn S, Setchell K D
Division of Gastroenterology, Medizinische Hochschule, Hannover, Federal Republic of Germany.
Biomed Chromatogr. 1986 Apr;1(2):89-92. doi: 10.1002/bmc.1130010207.
Enterolactone, enterodiol and secoisolariciresinol were conjugated with glucuronic acid by solubilized rabbit liver microsomal UDP-glucuronyltrasnferase. The monoglucuronide conjugate of all three substrates was formed and its identity confirmed by nuclear magnetic resonance (NMR) spectroscopy. Analytical high pressure liquid chromatography (HPLC) and NMR spectroscopy indicated conjugation with glucuronic acid to occur at several positions in the molecule. The enzymatic conjugation was monitored by analytical capillary isotachophoresis (ITP). The Km-values for enterlactone, enterodiol, and secoisolariciresinol were calculated to be 0.30, 0.23, and 0.22 mmol/l respectively.
肠内酯、肠二醇和开环异落叶松脂素通过溶解的兔肝微粒体UDP-葡萄糖醛酸基转移酶与葡萄糖醛酸结合。所有三种底物的单葡萄糖醛酸结合物均已形成，其结构通过核磁共振（NMR）光谱得以确认。分析型高压液相色谱（HPLC）和NMR光谱表明，葡萄糖醛酸结合发生在分子中的多个位置。通过分析型毛细管等速电泳（ITP）监测酶促结合反应。计算得出肠内酯、肠二醇和开环异落叶松脂素的Km值分别为0.30、0.23和0.22 mmol/l。
