Johnson L P, Fenselau C
Drug Metab Dispos. 1978 Nov-Dec;6(6):677-9.
Introduction of 18O into the acetal linkage of isoborneol glucuronide has been accomplished through the in vitro enzymatic conjugation of 18O-labeled isoborneol by use of immobilized, partially purified, rabbit liver UDP-glucuronyltransferase supplemented with UDP-glucuronic acid. Mass-spectrometric analysis of 18O enrichment in both the aglycon and the intact conjugate made it possible to study both enzymatic conjugation and enzymatic hydrolysis. Analysis by gas chromatography-mass spectrometry indicated that the labeled hydroxyl oxygen of isoborneol is retained during both conjugation and hydrolysis.
通过使用固定化的、部分纯化的兔肝UDP - 葡萄糖醛酸基转移酶并补充UDP - 葡萄糖醛酸，将18O引入异龙脑葡萄糖醛酸苷的缩醛键中，这一过程已在体外酶促结合18O标记的异龙脑时完成。对苷元和完整缀合物中18O富集情况的质谱分析使得研究酶促结合和酶促水解成为可能。气相色谱 - 质谱分析表明，异龙脑标记的羟基氧在结合和水解过程中均得以保留。
