ECERIFERUM11/C-末端结构域磷酸酶样 2 影响分泌性转运。
ECERIFERUM11/C-TERMINAL DOMAIN PHOSPHATASE-LIKE2 Affects Secretory Trafficking.
机构信息
Department of Botany, University of British Columbia, Vancouver, British Columbia, V6T 1Z1, Canada.
Department of Botany, University of British Columbia, Vancouver, British Columbia, V6T 1Z1, Canada
出版信息
Plant Physiol. 2019 Nov;181(3):901-915. doi: 10.1104/pp.19.00722. Epub 2019 Sep 4.
Abstract
Secretory trafficking is highly conserved in all eukaryotic cells and is required for secretion of proteins as well as extracellular matrix components. In plants, the export of cuticular waxes and various cell wall components relies on secretory trafficking, but the molecular mechanisms underlying their secretion are not well understood. In this study, we characterize the Arabidopsis () dwarf () mutant and we show that it exhibits reduced stem cuticular wax deposition, aberrant seed coat mucilage extrusion, and delayed secondary cell wall columella formation, as well as a block in secretory GFP trafficking. Cloning of the gene revealed that it encodes a C-TERMINAL DOMAIN PHOSPHATASE-LIKE2 (CPL2) protein. Thus, secretory trafficking in plant cells in general, and secretion of extracellular matrix constituents in developing epidermal cells in particular, involves a dephosphorylation step catalyzed by CER11/CPL2.
摘要
分泌运输在所有真核细胞中都高度保守，是蛋白质以及细胞外基质成分分泌所必需的。在植物中，角质层蜡质和各种细胞壁成分的输出依赖于分泌运输，但它们分泌的分子机制尚不清楚。在这项研究中，我们对拟南芥 ()矮化突变体进行了表征，结果表明它表现出茎部角质层蜡质沉积减少、种皮粘液异常挤出、次生细胞壁柱状结构形成延迟以及分泌 GFP 运输受阻。 dwarf 基因的克隆表明它编码一个 C-末端结构域磷酸酶样 2（CPL2）蛋白。因此，植物细胞的分泌运输，特别是发育中表皮细胞的细胞外基质成分的分泌，涉及由 CER11/CPL2 催化的去磷酸化步骤。
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