Department of Laboratory Medicine, The Jikei University Kashiwa Hospital.
Division of Cardiovascular and Internal Medicine, Kanazawa University Graduate School of Medical Sciences.
J Atheroscler Thromb. 2020 May 1;27(5):409-417. doi: 10.5551/jat.50187. Epub 2019 Sep 5.
The present study was conducted to establish a practical method for measuring non-cholesterol sterols and reference intervals of serum levels.
Healthy subjects (109 men and 151 women), four patients with sitosterolemia, and 10 heterozygous mutation carriers of ABCG5/ABCG8 genes were investigated. Then, three non-cholesterol sterols (sitosterol, campesterol, and lathosterol) of fasting serum samples were measured via a practical and highly sensitive gas chromatography (GC) method with 0.2 µg/mL as the lower limit of quantification. The coefficient of variation (CV) values for within-run reproducibility were 3.06%, 1.89%, and 1.77% for lathosterol, campesterol, and sitosterol, respectively. The CV values for between-run reproducibility were 2.81%, 2.06%, and 2.10% for lathosterol, campesterol, and sitosterol, respectively.
The serum levels of sitosterol and campesterol were significantly higher in women than in men, whereas the serum levels of lathosterol were significantly higher in men than in women. Because of these gender difference, the determination of reference intervals of the three sterol values was performed by considering gender. The reference intervals of sitosterol, campesterol, and lathosterol were 0.99-3.88, 2.14-7.43, and 0.77-3.60 µg/mL in men and 1.03-4.45, 2.19-8.34, and 0.64-2.78 µg/mL in women, respectively. The serum levels of sitosterol and campesterol were higher in patients with sitosterolemia (94.3±47.3 and 66.3±36.6 µg/mL, respectively) than in healthy subjects.
These results demonstrate a practical and highly sensitive GC method to measure non-cholesterol sterol levels and gender-segregated reference intervals of sitosterol, campesterol, and lathosterol in Japanese healthy subjects.
本研究旨在建立一种实用的方法来测量非胆固醇甾醇并确定其血清水平的参考区间。
本研究共纳入 109 名男性和 151 名女性健康受试者、4 名甾醇血症患者和 10 名 ABCG5/ABCG8 基因突变杂合携带者。然后，采用一种实用且高度敏感的气相色谱（GC）方法测量空腹血清样本中的三种非胆固醇甾醇（豆甾醇、菜油甾醇和谷甾醇），其定量下限为 0.2 µg/mL。内重复性的变异系数（CV）值分别为 3.06%、1.89%和 1.77%，适用于谷甾醇、菜油甾醇和豆甾醇。批间重复性的 CV 值分别为 2.81%、2.06%和 2.10%，适用于谷甾醇、菜油甾醇和豆甾醇。
女性的血清豆甾醇和菜油甾醇水平明显高于男性，而男性的血清谷甾醇水平明显高于女性。由于存在这种性别差异，因此通过考虑性别来确定这三种甾醇值的参考区间。男性的血清豆甾醇、菜油甾醇和谷甾醇的参考区间分别为 0.99-3.88、2.14-7.43 和 0.77-3.60 µg/mL，女性的血清豆甾醇、菜油甾醇和谷甾醇的参考区间分别为 1.03-4.45、2.19-8.34 和 0.64-2.78 µg/mL。甾醇血症患者的血清豆甾醇和菜油甾醇水平（分别为 94.3±47.3 和 66.3±36.6 µg/mL）明显高于健康受试者。
本研究建立了一种实用且高度敏感的 GC 方法，用于测量日本健康受试者的非胆固醇甾醇水平以及豆甾醇、菜油甾醇和谷甾醇的性别特异性参考区间。
