Institut für Klinische Chemie und Klinische Pharmakologie, Universitätsklinikum Bonn, Germany.
Klinik für Innere Medizin I, Universitätsklinikum Jena, Germany; Universitätsklinik für Innere Medizin - Kardiologie, Klinikum Oldenburg, Carl von Ossietzky Universität Oldenburg, Germany, European Medical School Oldenburg-Groningen, Germany.
Atherosclerosis. 2018 Dec;279:17-22. doi: 10.1016/j.atherosclerosis.2018.10.010. Epub 2018 Oct 17.
There are safety issues regarding plant sterol ester-enriched functional food. Oxidized plant sterols, also called oxyphytosterols, are supposed to contribute to plant sterol atherogenicity. This study aimed to analyze associations of plasma oxyphytosterol levels with cardiovascular events.
Plasma cholesterol was measured by gas chromatography-flame ionization detection. Plasma campesterol and sitosterol and their 7-oxygenated metabolites were analyzed by gas chromatography-mass selective detection.
In 376 patients admitted for elective coronary angiography, who were not on lipid-lowering drugs, 82 cardiovascular events occurred during a follow-up period of 4.2 ± 1.8 years. Patients with cardiovascular events had significantly higher 7α-hydroxycampesterol plasma levels (median, 0.46; [interquartile range (IQR) 0.22-0.81] nmol/L vs. median, 0.25 [IQR, 0.17-0.61] nmol/L; p = 0.003) and 7α-hydroxycampesterol-to-cholesterol ratios (median 0.08 [IQR, 0.04-0.14] nmol/mmol vs. median, 0.05 [IQR 0.03-0.11] nmol/mmol; p = 0.005) than controls without such events. Patients above the median were characterized by higher cumulative event rates in Kaplan-Meier-analysis (Logrank-test p = 0.084 and p = 0.025) for absolute and cholesterol corrected 7α-hydroxycampesterol, respectively. After adjustment for influencing factors and related lipids, the hazard ratios per one standard deviation of the log-transformed variables (HR) were 1.19 [95% confidence interval (CI), 0.95-1.48], p = 0.132 for 7α-hydroxycampesterol and HR, 1.18 [95% CI, 0.94-1.48], p = 0.154 for 7α-hydroxycampesterol-to-cholesterol ratio. None of the other investigated oxyphytosterols showed an association with cardiovascular events.
In patients not on lipid-lowering drugs, absolute plasma levels of 7α-hydroxycampesterol and their ratios to cholesterol are associated with cardiovascular events. Further research is required to elucidate the role of OPS in cardiovascular diseases.
富含植物固醇酯的功能性食品存在安全问题。氧化植物固醇，也称为氧杂植物固醇，据推测会导致植物固醇的动脉粥样硬化。本研究旨在分析血浆氧杂植物固醇水平与心血管事件的关系。
采用气相色谱-火焰离子化检测法测定血浆胆固醇。采用气相色谱-质谱选择检测法分析血浆菜油固醇和谷固醇及其 7-氧代代谢物。
在 376 名因选择性冠状动脉造影入院且未服用降脂药物的患者中，在 4.2±1.8 年的随访期间发生了 82 例心血管事件。与无心血管事件的患者相比，发生心血管事件的患者的 7α-羟基菜油固醇血浆水平（中位数，0.46；[四分位距（IQR）0.22-0.81]nmol/L）和 7α-羟基菜油固醇/胆固醇比值（中位数 0.08；[IQR 0.04-0.14]nmol/mmol）明显更高（中位数 0.25；[IQR 0.17-0.61]nmol/L；p=0.003）。在 Kaplan-Meier 分析中，超过中位数的患者具有更高的累积事件率（对数秩检验 p=0.084 和 p=0.025），分别用于绝对和胆固醇校正的 7α-羟基菜油固醇。在校正影响因素和相关脂质后，对数转换变量的一个标准差的危害比（HR）分别为 1.19（95%置信区间（CI），0.95-1.48），p=0.132 为 7α-羟基菜油固醇，HR 为 1.18（95%CI，0.94-1.48），p=0.154 为 7α-羟基菜油固醇/胆固醇比值。其他研究的氧杂植物固醇均与心血管事件无关。
在未服用降脂药物的患者中，血浆 7α-羟基菜油固醇的绝对值及其与胆固醇的比值与心血管事件相关。需要进一步研究来阐明 OPS 在心血管疾病中的作用。
