Department of Obstetrics, First People's Hospital of Jining, Jining, Shandong 272000, China.
Department of Nursing, First People's Hospital of Jining, Jining, Shandong 272000, China.
Comput Math Methods Med. 2019 Aug 14;2019:6936175. doi: 10.1155/2019/6936175. eCollection 2019.
Gestational diabetes mellitus (GDM) refers to the condition which shows abnormal glucose metabolism that occurs during pregnancy, while normal glucose metabolism before pregnancy. In the present study, a novel analytical procedure was used to explore the key molecule of gestational diabetes mellitus. First, the weighted pathway model was carried out subsequently to eliminate the gene-overlapping effects among pathways. Second, we assessed the enriched pathways by a combination of Fisher's -test and the Mann-Whitney test. We carried out the functional principal component analysis by estimating values of genes to identify the hub genes in the enriched pathways. Results showed that a total of 4 differential pathways were enriched. The key pathway was considered as the insulin secretion pathway. values of each gene in the key pathway were calculated. Three hub molecules were identified as hub differentially methylated genes, namely, , , and . In addition, by further comparing the gene expression data in a validation cohort, one key molecule was obtained, . Therefore, may serve as a potential target for the treatment of GDM.
妊娠期糖尿病（GDM）是指妊娠期间出现的葡萄糖代谢异常，而妊娠前的葡萄糖代谢正常。在本研究中，采用了一种新的分析方法来探索妊娠期糖尿病的关键分子。首先，进行加权通路模型分析，以消除通路之间的基因重叠效应。其次，我们采用 Fisher 检验和 Mann-Whitney 检验相结合的方法评估富集通路。我们通过估计基因的 值进行功能主成分分析，以识别富集通路中的枢纽基因。结果表明，共有 4 个差异通路被富集。关键通路被认为是胰岛素分泌通路。计算了关键通路中每个基因的 值。鉴定出 3 个枢纽分子作为枢纽差异甲基化基因，即 、 、和 。此外，通过进一步比较验证队列中的基因表达数据，获得了一个关键分子 。因此， 可能成为治疗 GDM 的潜在靶点。
